Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13103 | 3' | -66 | NC_003401.1 | + | 43318 | 0.66 | 0.443656 |
Target: 5'- gCUGGUCGCC-GGCGuucccgugaGGCG-GGcCGCg -3' miRNA: 3'- aGACCGGCGGuCCGCc--------CCGCaCC-GCG- -5' |
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13103 | 3' | -66 | NC_003401.1 | + | 115734 | 0.66 | 0.441941 |
Target: 5'- -gUGGCU-CCGGGUGGGGgCGgcucggcuccggGGUGCu -3' miRNA: 3'- agACCGGcGGUCCGCCCC-GCa-----------CCGCG- -5' |
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13103 | 3' | -66 | NC_003401.1 | + | 132649 | 0.66 | 0.43512 |
Target: 5'- cUCcGcGCCGCCGGGaggGGGGCc-GGgGCc -3' miRNA: 3'- -AGaC-CGGCGGUCCg--CCCCGcaCCgCG- -5' |
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13103 | 3' | -66 | NC_003401.1 | + | 30361 | 0.66 | 0.43512 |
Target: 5'- gUUGaGgCGCC-GGUGGGGCGaucugGGUGUg -3' miRNA: 3'- aGAC-CgGCGGuCCGCCCCGCa----CCGCG- -5' |
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13103 | 3' | -66 | NC_003401.1 | + | 21820 | 0.66 | 0.418337 |
Target: 5'- --aGGCaGCuUGGGCGGGGCcgaccacaGGCGCg -3' miRNA: 3'- agaCCGgCG-GUCCGCCCCGca------CCGCG- -5' |
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13103 | 3' | -66 | NC_003401.1 | + | 76607 | 0.66 | 0.410095 |
Target: 5'- cUCUGGCauuuaCGCCaagGGGCGauGCGUGcGCGUa -3' miRNA: 3'- -AGACCG-----GCGG---UCCGCccCGCAC-CGCG- -5' |
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13103 | 3' | -66 | NC_003401.1 | + | 27600 | 0.67 | 0.397119 |
Target: 5'- --gGGCacgCGCCAGcGCGGGGgGcagaggcugaaggaaUGGCGUu -3' miRNA: 3'- agaCCG---GCGGUC-CGCCCCgC---------------ACCGCG- -5' |
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13103 | 3' | -66 | NC_003401.1 | + | 14172 | 0.67 | 0.385984 |
Target: 5'- aUUGGCCGCgcggcucuggCGGGCGGcaaguacaGGCGUgugGGUGUg -3' miRNA: 3'- aGACCGGCG----------GUCCGCC--------CCGCA---CCGCG- -5' |
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13103 | 3' | -66 | NC_003401.1 | + | 20594 | 0.67 | 0.385984 |
Target: 5'- --cGGCCGCCAacccccaacGCGGGGCc--GCGCc -3' miRNA: 3'- agaCCGGCGGUc--------CGCCCCGcacCGCG- -5' |
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13103 | 3' | -66 | NC_003401.1 | + | 27708 | 0.67 | 0.385984 |
Target: 5'- --nGGCuCGCCGGcuGCGGGGgCgGUGGuUGCg -3' miRNA: 3'- agaCCG-GCGGUC--CGCCCC-G-CACC-GCG- -5' |
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13103 | 3' | -66 | NC_003401.1 | + | 69737 | 0.67 | 0.370439 |
Target: 5'- --cGGCCGCCAuGCGGGacucuggaGUGuUGGCGa -3' miRNA: 3'- agaCCGGCGGUcCGCCC--------CGC-ACCGCg -5' |
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13103 | 3' | -66 | NC_003401.1 | + | 32319 | 0.67 | 0.370439 |
Target: 5'- -gUGcCCGCCuaaaaGGGCGGuGGUgccgggcgGUGGCGCg -3' miRNA: 3'- agACcGGCGG-----UCCGCC-CCG--------CACCGCG- -5' |
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13103 | 3' | -66 | NC_003401.1 | + | 121358 | 0.68 | 0.347203 |
Target: 5'- cCUGGCCgagacgGCCcGGUGGGGUggucuguGUGGCu- -3' miRNA: 3'- aGACCGG------CGGuCCGCCCCG-------CACCGcg -5' |
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13103 | 3' | -66 | NC_003401.1 | + | 23765 | 0.68 | 0.340655 |
Target: 5'- uUUUGGCCugcuGCCAGGUGGcagcCGUccaGGCGCg -3' miRNA: 3'- -AGACCGG----CGGUCCGCCcc--GCA---CCGCG- -5' |
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13103 | 3' | -66 | NC_003401.1 | + | 121931 | 0.68 | 0.340655 |
Target: 5'- --gGGgCGCgGGuGCGGcGGC-UGGCGCg -3' miRNA: 3'- agaCCgGCGgUC-CGCC-CCGcACCGCG- -5' |
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13103 | 3' | -66 | NC_003401.1 | + | 8859 | 0.68 | 0.340655 |
Target: 5'- cCUGGgguacuUCGCgGGGCGcGGCGUGGacCGCa -3' miRNA: 3'- aGACC------GGCGgUCCGCcCCGCACC--GCG- -5' |
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13103 | 3' | -66 | NC_003401.1 | + | 18184 | 0.68 | 0.333485 |
Target: 5'- --cGGCCGCC-GGUGuGGGUG-GGCa- -3' miRNA: 3'- agaCCGGCGGuCCGC-CCCGCaCCGcg -5' |
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13103 | 3' | -66 | NC_003401.1 | + | 18141 | 0.68 | 0.312643 |
Target: 5'- --cGGCCGCU---CGGGGCGaucUGGCGUu -3' miRNA: 3'- agaCCGGCGGuccGCCCCGC---ACCGCG- -5' |
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13103 | 3' | -66 | NC_003401.1 | + | 23483 | 0.69 | 0.292804 |
Target: 5'- cUCgGGgaGCCGGGUGcaGGGCGggGGCGUg -3' miRNA: 3'- -AGaCCggCGGUCCGC--CCCGCa-CCGCG- -5' |
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13103 | 3' | -66 | NC_003401.1 | + | 132536 | 0.69 | 0.286413 |
Target: 5'- --cGGCCcggcGCgCGGcGCGGGGCGgccgGGgGCg -3' miRNA: 3'- agaCCGG----CG-GUC-CGCCCCGCa---CCgCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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