Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13107 | 5' | -55.3 | NC_003401.1 | + | 121446 | 0.66 | 0.926406 |
Target: 5'- gGGGUgGUCcCGGUGAgUCGGGCGgGg- -3' miRNA: 3'- -CCCAaCAGcGCCACU-GGCUUGCgCau -5' |
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13107 | 5' | -55.3 | NC_003401.1 | + | 22196 | 0.66 | 0.926406 |
Target: 5'- cGG-UGUUGCGGUuGCCuuuGAGCGCGc- -3' miRNA: 3'- cCCaACAGCGCCAcUGG---CUUGCGCau -5' |
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13107 | 5' | -55.3 | NC_003401.1 | + | 125411 | 0.66 | 0.920919 |
Target: 5'- cGGGcgUGUCGUgaggGGUcucucuaagaaGACCGAGCGCc-- -3' miRNA: 3'- -CCCa-ACAGCG----CCA-----------CUGGCUUGCGcau -5' |
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13107 | 5' | -55.3 | NC_003401.1 | + | 49240 | 0.66 | 0.920919 |
Target: 5'- -aGUgGUCGCGGUGuauacCCGAacaaACGCGg- -3' miRNA: 3'- ccCAaCAGCGCCACu----GGCU----UGCGCau -5' |
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13107 | 5' | -55.3 | NC_003401.1 | + | 70154 | 0.66 | 0.920919 |
Target: 5'- cGGGUUG-CGUGG-GACugucuCGGugGUGUGa -3' miRNA: 3'- -CCCAACaGCGCCaCUG-----GCUugCGCAU- -5' |
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13107 | 5' | -55.3 | NC_003401.1 | + | 82547 | 0.67 | 0.860967 |
Target: 5'- gGGGgcgGUUGCGGUccugucGugCGccAACGCGUGu -3' miRNA: 3'- -CCCaa-CAGCGCCA------CugGC--UUGCGCAU- -5' |
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13107 | 5' | -55.3 | NC_003401.1 | + | 8293 | 0.68 | 0.845226 |
Target: 5'- aGGGacacuuaCGUGGUGGCCGGAC-CGUAc -3' miRNA: 3'- -CCCaaca---GCGCCACUGGCUUGcGCAU- -5' |
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13107 | 5' | -55.3 | NC_003401.1 | + | 22990 | 0.68 | 0.837059 |
Target: 5'- aGGGUUGuaauuUCGCGGggcccuuugGGCCccGCGCGUc -3' miRNA: 3'- -CCCAAC-----AGCGCCa--------CUGGcuUGCGCAu -5' |
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13107 | 5' | -55.3 | NC_003401.1 | + | 117926 | 0.69 | 0.811462 |
Target: 5'- cGGcGggGUCGCGGcGAggucacgccgaaCCGGGCGCGg- -3' miRNA: 3'- -CC-CaaCAGCGCCaCU------------GGCUUGCGCau -5' |
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13107 | 5' | -55.3 | NC_003401.1 | + | 89164 | 0.69 | 0.784389 |
Target: 5'- uGGGaacagUGUCGCcgggaacgGGUGACCGGGCGaGUc -3' miRNA: 3'- -CCCa----ACAGCG--------CCACUGGCUUGCgCAu -5' |
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13107 | 5' | -55.3 | NC_003401.1 | + | 28367 | 0.69 | 0.765636 |
Target: 5'- cGGG-UGUCGCGGUuaccgcaguGCUGAGCGCa-- -3' miRNA: 3'- -CCCaACAGCGCCAc--------UGGCUUGCGcau -5' |
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13107 | 5' | -55.3 | NC_003401.1 | + | 14205 | 0.69 | 0.765636 |
Target: 5'- aGGG-UGUCGgGGaccGGCCGggUGCGc- -3' miRNA: 3'- -CCCaACAGCgCCa--CUGGCuuGCGCau -5' |
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13107 | 5' | -55.3 | NC_003401.1 | + | 40798 | 0.71 | 0.666176 |
Target: 5'- cGGGUUGUgcaugCGCGGaUGACagauggacuUGAGCGCGUu -3' miRNA: 3'- -CCCAACA-----GCGCC-ACUG---------GCUUGCGCAu -5' |
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13107 | 5' | -55.3 | NC_003401.1 | + | 18928 | 1.09 | 0.003274 |
Target: 5'- uGGGUUGUCGCGGUGACCGAACGCGUAg -3' miRNA: 3'- -CCCAACAGCGCCACUGGCUUGCGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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