Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13109 | 5' | -53 | NC_003401.1 | + | 132679 | 0.66 | 0.968613 |
Target: 5'- cGggGCG-CCUCGGCGgggcccGGCGCGGg- -3' miRNA: 3'- -CuuUGUgGGAGCUGCau----CUGCGUCga -5' |
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13109 | 5' | -53 | NC_003401.1 | + | 118730 | 0.66 | 0.968613 |
Target: 5'- --cGCGCCgCUUGGCG---GCGCGGCUu -3' miRNA: 3'- cuuUGUGG-GAGCUGCaucUGCGUCGA- -5' |
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13109 | 5' | -53 | NC_003401.1 | + | 39762 | 0.66 | 0.968613 |
Target: 5'- ---cCGCCUcucuccaguUUGGCGUGGACGC-GCUg -3' miRNA: 3'- cuuuGUGGG---------AGCUGCAUCUGCGuCGA- -5' |
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13109 | 5' | -53 | NC_003401.1 | + | 94491 | 0.66 | 0.958233 |
Target: 5'- -uGGCGCUCUCGGCGUcaAgGUAGCc -3' miRNA: 3'- cuUUGUGGGAGCUGCAucUgCGUCGa -5' |
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13109 | 5' | -53 | NC_003401.1 | + | 109901 | 0.66 | 0.954304 |
Target: 5'- --uACACaaaCUCGAgaUGaUAGACGCAGCc -3' miRNA: 3'- cuuUGUGg--GAGCU--GC-AUCUGCGUCGa -5' |
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13109 | 5' | -53 | NC_003401.1 | + | 132217 | 0.66 | 0.954304 |
Target: 5'- gGAGGCGCCCggcCGGCGccgccgccgGGcCGCGGCc -3' miRNA: 3'- -CUUUGUGGGa--GCUGCa--------UCuGCGUCGa -5' |
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13109 | 5' | -53 | NC_003401.1 | + | 28381 | 0.66 | 0.950133 |
Target: 5'- ---cCACCCUCGAUGccGGCaacgGCGGCg -3' miRNA: 3'- cuuuGUGGGAGCUGCauCUG----CGUCGa -5' |
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13109 | 5' | -53 | NC_003401.1 | + | 132684 | 0.67 | 0.941048 |
Target: 5'- uGggGCcccggGCCCggggGGCGUcgGGGCGCGGCg -3' miRNA: 3'- -CuuUG-----UGGGag--CUGCA--UCUGCGUCGa -5' |
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13109 | 5' | -53 | NC_003401.1 | + | 74665 | 0.67 | 0.936129 |
Target: 5'- cGAGUAUCUUCGuCGUuuuGACGCGGCUa -3' miRNA: 3'- cUUUGUGGGAGCuGCAu--CUGCGUCGA- -5' |
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13109 | 5' | -53 | NC_003401.1 | + | 133650 | 0.68 | 0.913911 |
Target: 5'- cGGACGCCCUCG-C----ACGCAGCUg -3' miRNA: 3'- cUUUGUGGGAGCuGcaucUGCGUCGA- -5' |
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13109 | 5' | -53 | NC_003401.1 | + | 935 | 0.68 | 0.913911 |
Target: 5'- cGGACGCCCUCG-C----ACGCAGCUg -3' miRNA: 3'- cUUUGUGGGAGCuGcaucUGCGUCGA- -5' |
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13109 | 5' | -53 | NC_003401.1 | + | 20280 | 0.68 | 0.913911 |
Target: 5'- cGAcGCGCCCgggGACGcGGACGCcGCg -3' miRNA: 3'- -CUuUGUGGGag-CUGCaUCUGCGuCGa -5' |
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13109 | 5' | -53 | NC_003401.1 | + | 79757 | 0.68 | 0.894595 |
Target: 5'- aGAAAgACCgCUCGAUGcAGAUaGCGGCa -3' miRNA: 3'- -CUUUgUGG-GAGCUGCaUCUG-CGUCGa -5' |
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13109 | 5' | -53 | NC_003401.1 | + | 22357 | 0.69 | 0.887663 |
Target: 5'- aGAAACACCaUUaACGcAGACGCGGCc -3' miRNA: 3'- -CUUUGUGGgAGcUGCaUCUGCGUCGa -5' |
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13109 | 5' | -53 | NC_003401.1 | + | 44008 | 0.69 | 0.879761 |
Target: 5'- --uACGCCCUgGACGUGGACcccguugggucgaGCucAGCUa -3' miRNA: 3'- cuuUGUGGGAgCUGCAUCUG-------------CG--UCGA- -5' |
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13109 | 5' | -53 | NC_003401.1 | + | 18284 | 0.69 | 0.865446 |
Target: 5'- aGAGACACCCUUGugGccGcCGCcGCg -3' miRNA: 3'- -CUUUGUGGGAGCugCauCuGCGuCGa -5' |
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13109 | 5' | -53 | NC_003401.1 | + | 72601 | 0.7 | 0.844558 |
Target: 5'- aAGACGCCCUCGccggaauuaguauccGCGUGGACGUg--- -3' miRNA: 3'- cUUUGUGGGAGC---------------UGCAUCUGCGucga -5' |
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13109 | 5' | -53 | NC_003401.1 | + | 8911 | 0.7 | 0.832727 |
Target: 5'- cAGGCGCCCUgUGGCGgcGAC-CGGCUg -3' miRNA: 3'- cUUUGUGGGA-GCUGCauCUGcGUCGA- -5' |
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13109 | 5' | -53 | NC_003401.1 | + | 15037 | 0.71 | 0.787532 |
Target: 5'- --uGCGCCaCgcgCGACGgcGACGCGGUg -3' miRNA: 3'- cuuUGUGG-Ga--GCUGCauCUGCGUCGa -5' |
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13109 | 5' | -53 | NC_003401.1 | + | 91425 | 0.75 | 0.550758 |
Target: 5'- aGGGACGCCUcCGACGUGuacgugcccauGGCGCGGCg -3' miRNA: 3'- -CUUUGUGGGaGCUGCAU-----------CUGCGUCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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