Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13110 | 3' | -56.9 | NC_003401.1 | + | 96192 | 0.66 | 0.859666 |
Target: 5'- -cACCGAAACUUcuuuCUCUGCGGCa- -3' miRNA: 3'- gaUGGCUUUGGGcac-GAGACGCCGgu -5' |
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13110 | 3' | -56.9 | NC_003401.1 | + | 20289 | 0.66 | 0.851857 |
Target: 5'- gCUGCUGGAcgacgcGCCCGgggacGCggacgCcGCGGCCAu -3' miRNA: 3'- -GAUGGCUU------UGGGCa----CGa----GaCGCCGGU- -5' |
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13110 | 3' | -56.9 | NC_003401.1 | + | 23792 | 0.66 | 0.851857 |
Target: 5'- cCUGCCGGccgcucgcggcGCCCGUGUUuuuggcCUGCuGCCAg -3' miRNA: 3'- -GAUGGCUu----------UGGGCACGA------GACGcCGGU- -5' |
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13110 | 3' | -56.9 | NC_003401.1 | + | 132347 | 0.66 | 0.843846 |
Target: 5'- -cGCCG--GCCCGgGC-CcGCGGCCGc -3' miRNA: 3'- gaUGGCuuUGGGCaCGaGaCGCCGGU- -5' |
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13110 | 3' | -56.9 | NC_003401.1 | + | 16860 | 0.66 | 0.838946 |
Target: 5'- -aGCCGGcAGCCCcuccucguauaccgcGUGCg--GCGGCCGc -3' miRNA: 3'- gaUGGCU-UUGGG---------------CACGagaCGCCGGU- -5' |
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13110 | 3' | -56.9 | NC_003401.1 | + | 104666 | 0.66 | 0.8264 |
Target: 5'- -aACCGAAaacguuccggcgcGCCCGgcgaugGaCUCgGUGGCCAg -3' miRNA: 3'- gaUGGCUU-------------UGGGCa-----C-GAGaCGCCGGU- -5' |
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13110 | 3' | -56.9 | NC_003401.1 | + | 10452 | 0.66 | 0.818677 |
Target: 5'- -gACCGGAucccauagaAUCCcUGUUCUGCGGCg- -3' miRNA: 3'- gaUGGCUU---------UGGGcACGAGACGCCGgu -5' |
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13110 | 3' | -56.9 | NC_003401.1 | + | 132669 | 0.66 | 0.818677 |
Target: 5'- -gGCCGggGCCCGgggcGCcUCggcGgGGCCc -3' miRNA: 3'- gaUGGCuuUGGGCa---CG-AGa--CgCCGGu -5' |
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13110 | 3' | -56.9 | NC_003401.1 | + | 111528 | 0.68 | 0.763951 |
Target: 5'- -gACCGGcgUCCG-GgUgUGCGGCCAg -3' miRNA: 3'- gaUGGCUuuGGGCaCgAgACGCCGGU- -5' |
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13110 | 3' | -56.9 | NC_003401.1 | + | 107728 | 0.68 | 0.744666 |
Target: 5'- -cACCGggGCaac-GCUCgggGCGGCCGa -3' miRNA: 3'- gaUGGCuuUGggcaCGAGa--CGCCGGU- -5' |
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13110 | 3' | -56.9 | NC_003401.1 | + | 52451 | 0.68 | 0.715015 |
Target: 5'- -gACUGGAcCgCCGUGCUCggacGCGGCUc -3' miRNA: 3'- gaUGGCUUuG-GGCACGAGa---CGCCGGu -5' |
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13110 | 3' | -56.9 | NC_003401.1 | + | 132545 | 0.69 | 0.704977 |
Target: 5'- -aACCGAAagcgGCCCGgcgcGCggcgCgggGCGGCCGg -3' miRNA: 3'- gaUGGCUU----UGGGCa---CGa---Ga--CGCCGGU- -5' |
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13110 | 3' | -56.9 | NC_003401.1 | + | 91389 | 0.7 | 0.633468 |
Target: 5'- gCUACCG--GCCUG-GCUCUcGCGGCa- -3' miRNA: 3'- -GAUGGCuuUGGGCaCGAGA-CGCCGgu -5' |
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13110 | 3' | -56.9 | NC_003401.1 | + | 78289 | 0.7 | 0.623181 |
Target: 5'- uCUG-CGGGACUCGagGCUCUcGCGGCCu -3' miRNA: 3'- -GAUgGCUUUGGGCa-CGAGA-CGCCGGu -5' |
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13110 | 3' | -56.9 | NC_003401.1 | + | 124419 | 0.71 | 0.551778 |
Target: 5'- -aGCCGGAGgcCCCGUGCUUgacccacggGCGGCgGg -3' miRNA: 3'- gaUGGCUUU--GGGCACGAGa--------CGCCGgU- -5' |
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13110 | 3' | -56.9 | NC_003401.1 | + | 23726 | 1.08 | 0.002134 |
Target: 5'- aCUACCGAAACCCGUGCUCUGCGGCCAc -3' miRNA: 3'- -GAUGGCUUUGGGCACGAGACGCCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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