Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13111 | 5' | -46.3 | NC_003401.1 | + | 84939 | 0.7 | 0.993408 |
Target: 5'- gGCCAAAcacuuuCUAauguccACCACUCaCUGCCGg -3' miRNA: 3'- -UGGUUUuuu---GAU------UGGUGAG-GAUGGCa -5' |
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13111 | 5' | -46.3 | NC_003401.1 | + | 42407 | 0.71 | 0.986666 |
Target: 5'- gGCCAGcauugGAGACUuguGCCACUCCUcguacucgGCCa- -3' miRNA: 3'- -UGGUU-----UUUUGAu--UGGUGAGGA--------UGGca -5' |
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13111 | 5' | -46.3 | NC_003401.1 | + | 27485 | 0.71 | 0.984837 |
Target: 5'- cGCCAGAAGACcAGCCGCaaCU-CCGUa -3' miRNA: 3'- -UGGUUUUUUGaUUGGUGagGAuGGCA- -5' |
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13111 | 5' | -46.3 | NC_003401.1 | + | 115424 | 0.74 | 0.954772 |
Target: 5'- aGCCAccccGGAGCcgAGCCGCUCCcACCGg -3' miRNA: 3'- -UGGUu---UUUUGa-UUGGUGAGGaUGGCa -5' |
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13111 | 5' | -46.3 | NC_003401.1 | + | 26818 | 1.09 | 0.022178 |
Target: 5'- uACCAAAAAACUAACCACUCCUACCGUg -3' miRNA: 3'- -UGGUUUUUUGAUUGGUGAGGAUGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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