Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13113 | 5' | -57.6 | NC_003401.1 | + | 17722 | 0.67 | 0.769548 |
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Target: 5'- -cCCUgCGCGUcucCGGCGAggcGUCUCAGa -3' miRNA: 3'- gaGGA-GCGCAac-GCCGCUa--CGGAGUC- -5' |
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| 13113 | 5' | -57.6 | NC_003401.1 | + | 42743 | 0.67 | 0.740842 |
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Target: 5'- -cCCgUCGCuuacGUUGCGGgGGUGCCgcacUCGGa -3' miRNA: 3'- gaGG-AGCG----CAACGCCgCUACGG----AGUC- -5' |
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| 13113 | 5' | -57.6 | NC_003401.1 | + | 42583 | 0.68 | 0.721215 |
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Target: 5'- -gCC-CGCccacGUUGCGGCGAUuaCCUCGGa -3' miRNA: 3'- gaGGaGCG----CAACGCCGCUAc-GGAGUC- -5' |
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| 13113 | 5' | -57.6 | NC_003401.1 | + | 127687 | 0.68 | 0.701283 |
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Target: 5'- -gUCUCGCGggGCGGCcugcGCCUCu- -3' miRNA: 3'- gaGGAGCGCaaCGCCGcua-CGGAGuc -5' |
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| 13113 | 5' | -57.6 | NC_003401.1 | + | 78246 | 0.69 | 0.670973 |
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Target: 5'- -aCCUCGgGUUGCagcgguGGCGgcGCCggCAGg -3' miRNA: 3'- gaGGAGCgCAACG------CCGCuaCGGa-GUC- -5' |
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| 13113 | 5' | -57.6 | NC_003401.1 | + | 118829 | 0.71 | 0.52921 |
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Target: 5'- -cCCUCGCGUUGCGGgGGUcauacgGCC-CAc -3' miRNA: 3'- gaGGAGCGCAACGCCgCUA------CGGaGUc -5' |
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| 13113 | 5' | -57.6 | NC_003401.1 | + | 27449 | 1.08 | 0.002138 |
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Target: 5'- uCUCCUCGCGUUGCGGCGAUGCCUCAGu -3' miRNA: 3'- -GAGGAGCGCAACGCCGCUACGGAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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