Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13119 | 3' | -62.5 | NC_003401.1 | + | 121314 | 0.67 | 0.530092 |
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Target: 5'- cGCGCCCcccgCGCCCCCCGcGcCcGcCGCg -3' miRNA: 3'- -CGUGGGua--GCGGGGGGC-CaGaCcGUG- -5' |
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| 13119 | 3' | -62.5 | NC_003401.1 | + | 20364 | 0.67 | 0.543517 |
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Target: 5'- -aACCCcacuucagacaggCGCCCCCUGGUCcugucaagagaGGCGCg -3' miRNA: 3'- cgUGGGua-----------GCGGGGGGCCAGa----------CCGUG- -5' |
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| 13119 | 3' | -62.5 | NC_003401.1 | + | 17507 | 0.66 | 0.558993 |
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Target: 5'- gGCGCgCGUgGCCCCCgGGUucgugCUGG-ACu -3' miRNA: 3'- -CGUGgGUAgCGGGGGgCCA-----GACCgUG- -5' |
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| 13119 | 3' | -62.5 | NC_003401.1 | + | 110742 | 0.66 | 0.568729 |
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Target: 5'- -uGCCUAUaCGCCCCCCGaUCaUGG-ACg -3' miRNA: 3'- cgUGGGUA-GCGGGGGGCcAG-ACCgUG- -5' |
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| 13119 | 3' | -62.5 | NC_003401.1 | + | 88356 | 0.66 | 0.588319 |
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Target: 5'- uGCucCCCGUCGCCUCCCac---GGCGCc -3' miRNA: 3'- -CGu-GGGUAGCGGGGGGccagaCCGUG- -5' |
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| 13119 | 3' | -62.5 | NC_003401.1 | + | 118021 | 0.66 | 0.608028 |
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Target: 5'- cGUACCCAUCGgCCgCUC--UUUGGCGCc -3' miRNA: 3'- -CGUGGGUAGCgGG-GGGccAGACCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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