Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13121 | 3' | -63 | NC_003401.1 | + | 18010 | 0.66 | 0.576573 |
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Target: 5'- -uUCGGGGAgaUCcGCGcGUCCCUGuGCGc -3' miRNA: 3'- ccAGCCCCU--AGaCGC-CAGGGGCcCGU- -5' |
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| 13121 | 3' | -63 | NC_003401.1 | + | 20116 | 0.66 | 0.566934 |
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Target: 5'- uGGcCGcGGcGUCcGC-GUCCCCGGGCGc -3' miRNA: 3'- -CCaGC-CCcUAGaCGcCAGGGGCCCGU- -5' |
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| 13121 | 3' | -63 | NC_003401.1 | + | 132514 | 0.68 | 0.464728 |
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Target: 5'- cGGcCGGGGG-CgGCGG-CCgCGGGCc -3' miRNA: 3'- -CCaGCCCCUaGaCGCCaGGgGCCCGu -5' |
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| 13121 | 3' | -63 | NC_003401.1 | + | 118449 | 0.69 | 0.438502 |
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Target: 5'- -cUCGGG--UCcGCGGgCCCCGGGCu -3' miRNA: 3'- ccAGCCCcuAGaCGCCaGGGGCCCGu -5' |
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| 13121 | 3' | -63 | NC_003401.1 | + | 132663 | 0.69 | 0.438502 |
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Target: 5'- cGUCGGGGc---GCGGcgCCgCCGGGCu -3' miRNA: 3'- cCAGCCCCuagaCGCCa-GG-GGCCCGu -5' |
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| 13121 | 3' | -63 | NC_003401.1 | + | 125392 | 0.71 | 0.335511 |
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Target: 5'- uGGUCGGGGGUUU-UGGg-UCCGGGCGu -3' miRNA: 3'- -CCAGCCCCUAGAcGCCagGGGCCCGU- -5' |
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| 13121 | 3' | -63 | NC_003401.1 | + | 132434 | 0.71 | 0.328359 |
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Target: 5'- cGGgccCGGGGggCgcGCGGgggacccucgCCCCGGGCGc -3' miRNA: 3'- -CCa--GCCCCuaGa-CGCCa---------GGGGCCCGU- -5' |
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| 13121 | 3' | -63 | NC_003401.1 | + | 132417 | 0.73 | 0.247907 |
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Target: 5'- gGGcCGGGGGUCccGCGGggggccggggccgcCCCCGGGUg -3' miRNA: 3'- -CCaGCCCCUAGa-CGCCa-------------GGGGCCCGu -5' |
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| 13121 | 3' | -63 | NC_003401.1 | + | 31370 | 1.09 | 0.000629 |
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Target: 5'- uGGUCGGGGAUCUGCGGUCCCCGGGCAg -3' miRNA: 3'- -CCAGCCCCUAGACGCCAGGGGCCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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