Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13125 | 3' | -48.1 | NC_003401.1 | + | 126678 | 0.66 | 0.998695 |
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Target: 5'- aUGUCGcuuagaCGCCGCACGUgcgcuagcauGAUGUUGg- -3' miRNA: 3'- -ACGGCaa----GUGGCGUGCA----------UUAUAACaa -5' |
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| 13125 | 3' | -48.1 | NC_003401.1 | + | 104069 | 0.66 | 0.998078 |
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Target: 5'- aGCCGUUCGgCCGC-CGaGAUGcUGg- -3' miRNA: 3'- aCGGCAAGU-GGCGuGCaUUAUaACaa -5' |
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| 13125 | 3' | -48.1 | NC_003401.1 | + | 109109 | 0.68 | 0.992693 |
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Target: 5'- gGCCGUUC-CCaggaGCGCGUGGUccagguaucuuGUUGUg -3' miRNA: 3'- aCGGCAAGuGG----CGUGCAUUA-----------UAACAa -5' |
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| 13125 | 3' | -48.1 | NC_003401.1 | + | 21777 | 0.68 | 0.992693 |
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Target: 5'- gGCCGUcCGCUGCGCGUGc------- -3' miRNA: 3'- aCGGCAaGUGGCGUGCAUuauaacaa -5' |
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| 13125 | 3' | -48.1 | NC_003401.1 | + | 31537 | 0.69 | 0.988838 |
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Target: 5'- -cCCGUuaagCGCCGCcgACGUGGUGUUGa- -3' miRNA: 3'- acGGCAa---GUGGCG--UGCAUUAUAACaa -5' |
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| 13125 | 3' | -48.1 | NC_003401.1 | + | 26776 | 0.72 | 0.94303 |
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Target: 5'- cUGCCGgUC-CCGCGCGUGGUAc---- -3' miRNA: 3'- -ACGGCaAGuGGCGUGCAUUAUaacaa -5' |
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| 13125 | 3' | -48.1 | NC_003401.1 | + | 47885 | 1.06 | 0.021237 |
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Target: 5'- cUGCCGUUCACCGCACGUAAUAUUGUUc -3' miRNA: 3'- -ACGGCAAGUGGCGUGCAUUAUAACAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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