Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13125 | 5' | -50.3 | NC_003401.1 | + | 14129 | 0.66 | 0.993516 |
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Target: 5'- cUGGGCCCUCGCGGAc----CACGc-- -3' miRNA: 3'- -AUUUGGGAGCGCCUucauaGUGCacu -5' |
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| 13125 | 5' | -50.3 | NC_003401.1 | + | 121179 | 0.66 | 0.993516 |
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Target: 5'- ---cCCCUCGgcCGGGcccguGGUGUCACG-GAg -3' miRNA: 3'- auuuGGGAGC--GCCU-----UCAUAGUGCaCU- -5' |
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| 13125 | 5' | -50.3 | NC_003401.1 | + | 18431 | 0.67 | 0.981424 |
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Target: 5'- ----gUCUCgGCGG-AGUGUCACGUGu -3' miRNA: 3'- auuugGGAG-CGCCuUCAUAGUGCACu -5' |
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| 13125 | 5' | -50.3 | NC_003401.1 | + | 78301 | 0.68 | 0.976695 |
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Target: 5'- gAGGCUCUCGCGGccuuuaucGAGgaagGUCugGCGUGGg -3' miRNA: 3'- aUUUGGGAGCGCC--------UUCa---UAG--UGCACU- -5' |
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| 13125 | 5' | -50.3 | NC_003401.1 | + | 42490 | 0.68 | 0.976695 |
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Target: 5'- aUGAACCCcggCGCGGAcAGcuUCAUGUGc -3' miRNA: 3'- -AUUUGGGa--GCGCCU-UCauAGUGCACu -5' |
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| 13125 | 5' | -50.3 | NC_003401.1 | + | 106707 | 0.68 | 0.971137 |
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Target: 5'- gAGGCCUgaggUGCGGuugacgguGUGUUACGUGAc -3' miRNA: 3'- aUUUGGGa---GCGCCuu------CAUAGUGCACU- -5' |
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| 13125 | 5' | -50.3 | NC_003401.1 | + | 47660 | 0.7 | 0.934366 |
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Target: 5'- -cGAUCCgUCGCGGGucAGUGcgacggguuguuUCACGUGAu -3' miRNA: 3'- auUUGGG-AGCGCCU--UCAU------------AGUGCACU- -5' |
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| 13125 | 5' | -50.3 | NC_003401.1 | + | 72597 | 0.77 | 0.602067 |
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Target: 5'- --cGCCCUCGcCGGAauuAGUAUCcGCGUGGa -3' miRNA: 3'- auuUGGGAGC-GCCU---UCAUAG-UGCACU- -5' |
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| 13125 | 5' | -50.3 | NC_003401.1 | + | 47851 | 1.08 | 0.009232 |
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Target: 5'- aUAAACCCUCGCGGAAGUAUCACGUGAa -3' miRNA: 3'- -AUUUGGGAGCGCCUUCAUAGUGCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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