Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13126 | 3' | -53.1 | NC_003401.1 | + | 127996 | 0.67 | 0.94433 |
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Target: 5'- ---aGGGCCGAUCUgauuucgcccccgGGCaGCAGCguGGCa -3' miRNA: 3'- cuagCCUGGUUAGA-------------UCGcCGUUG--CCG- -5' |
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| 13126 | 3' | -53.1 | NC_003401.1 | + | 128776 | 0.69 | 0.886483 |
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Target: 5'- --cUGGACUAAg--GGCuGCAGCGGCc -3' miRNA: 3'- cuaGCCUGGUUagaUCGcCGUUGCCG- -5' |
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| 13126 | 3' | -53.1 | NC_003401.1 | + | 128814 | 0.68 | 0.893431 |
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Target: 5'- cGUUGGGCCAuauuugcuaaGUCUAGCcGGCGcaaaagcaaGCGGg -3' miRNA: 3'- cUAGCCUGGU----------UAGAUCG-CCGU---------UGCCg -5' |
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| 13126 | 3' | -53.1 | NC_003401.1 | + | 132402 | 0.72 | 0.73756 |
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Target: 5'- --cCGGGCgCAAgaUGGCGGCcGCGGCc -3' miRNA: 3'- cuaGCCUG-GUUagAUCGCCGuUGCCG- -5' |
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| 13126 | 3' | -53.1 | NC_003401.1 | + | 132608 | 0.69 | 0.878567 |
|
Target: 5'- --cCGGGCCcggCggAGCGGCAcccggggGCGGCc -3' miRNA: 3'- cuaGCCUGGuuaGa-UCGCCGU-------UGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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