Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13128 | 3' | -50.4 | NC_003401.1 | + | 96693 | 0.66 | 0.994305 |
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Target: 5'- ----gGgGGGCAGaGUccGCGUcCAUCCu -3' miRNA: 3'- ugaaaCgCCUGUC-CAuaCGCA-GUAGG- -5' |
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| 13128 | 3' | -50.4 | NC_003401.1 | + | 49829 | 0.66 | 0.992368 |
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Target: 5'- --cUUGCGaGuCAGGUG-GCGUgUAUCCg -3' miRNA: 3'- ugaAACGC-CuGUCCAUaCGCA-GUAGG- -5' |
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| 13128 | 3' | -50.4 | NC_003401.1 | + | 78335 | 0.66 | 0.98994 |
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Target: 5'- ----cGUGGGCccAGGUGUGCG-UAUCUc -3' miRNA: 3'- ugaaaCGCCUG--UCCAUACGCaGUAGG- -5' |
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| 13128 | 3' | -50.4 | NC_003401.1 | + | 33234 | 0.67 | 0.988368 |
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Target: 5'- cACcgUUGCGGACaguAGGUGUcUGUCAaaaacaaUCCa -3' miRNA: 3'- -UGa-AACGCCUG---UCCAUAcGCAGU-------AGG- -5' |
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| 13128 | 3' | -50.4 | NC_003401.1 | + | 96414 | 0.67 | 0.986946 |
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Target: 5'- cGCgcagGCuGGACGGGcAUGaGUCAUCUa -3' miRNA: 3'- -UGaaa-CG-CCUGUCCaUACgCAGUAGG- -5' |
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| 13128 | 3' | -50.4 | NC_003401.1 | + | 83308 | 0.71 | 0.898144 |
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Target: 5'- ---cUGCGaGACAGGU---CGUCAUCCc -3' miRNA: 3'- ugaaACGC-CUGUCCAuacGCAGUAGG- -5' |
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| 13128 | 3' | -50.4 | NC_003401.1 | + | 14443 | 0.74 | 0.7712 |
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Target: 5'- cGCg--GCGGACAGGUGUGcCGagGUaCCu -3' miRNA: 3'- -UGaaaCGCCUGUCCAUAC-GCagUA-GG- -5' |
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| 13128 | 3' | -50.4 | NC_003401.1 | + | 51807 | 1.13 | 0.004573 |
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Target: 5'- cACUUUGCGGACAGGUAUGCGUCAUCCg -3' miRNA: 3'- -UGAAACGCCUGUCCAUACGCAGUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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