Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13128 | 5' | -56.8 | NC_003401.1 | + | 22774 | 0.69 | 0.711393 |
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Target: 5'- cCGAGcacgGCGAGCG-UGGCgcugGUG-GCAACg -3' miRNA: 3'- -GCUC----UGCUCGCuACCGa---CGCgCGUUG- -5' |
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| 13128 | 5' | -56.8 | NC_003401.1 | + | 27856 | 0.69 | 0.701317 |
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Target: 5'- aCGAGAacugaaCGGGCGGUGGUUGCGguCGUuggucGGCg -3' miRNA: 3'- -GCUCU------GCUCGCUACCGACGC--GCG-----UUG- -5' |
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| 13128 | 5' | -56.8 | NC_003401.1 | + | 132678 | 0.69 | 0.670769 |
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Target: 5'- cCGGGGCGccucGGCGG-GGCccgGCGCGgGGCg -3' miRNA: 3'- -GCUCUGC----UCGCUaCCGa--CGCGCgUUG- -5' |
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| 13128 | 5' | -56.8 | NC_003401.1 | + | 78243 | 0.7 | 0.619332 |
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Target: 5'- uCGGGuuGcAGCGGUGGCgGCGC-CGGCa -3' miRNA: 3'- -GCUCugC-UCGCUACCGaCGCGcGUUG- -5' |
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| 13128 | 5' | -56.8 | NC_003401.1 | + | 121930 | 0.71 | 0.568128 |
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Target: 5'- gGGGGCGcggguGCGgcGGCUG-GCGCGGCc -3' miRNA: 3'- gCUCUGCu----CGCuaCCGACgCGCGUUG- -5' |
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| 13128 | 5' | -56.8 | NC_003401.1 | + | 118114 | 0.72 | 0.537896 |
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Target: 5'- gGGGACG-GUGggGGCcGCGCGCcACc -3' miRNA: 3'- gCUCUGCuCGCuaCCGaCGCGCGuUG- -5' |
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| 13128 | 5' | -56.8 | NC_003401.1 | + | 13878 | 0.73 | 0.469821 |
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Target: 5'- gGGGGCcuGGGCGAUGGCguugGUGCGCc-- -3' miRNA: 3'- gCUCUG--CUCGCUACCGa---CGCGCGuug -5' |
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| 13128 | 5' | -56.8 | NC_003401.1 | + | 51768 | 1.09 | 0.002093 |
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Target: 5'- cCGAGACGAGCGAUGGCUGCGCGCAACc -3' miRNA: 3'- -GCUCUGCUCGCUACCGACGCGCGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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