Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1313 | 3' | -55.3 | NC_001331.1 | + | 6889 | 0.67 | 0.14764 |
Target: 5'- gACCGGaucGGCCAcggugcGGGCGGcuUGGaucAGGCa -3' miRNA: 3'- -UGGCUa--CCGGU------CCCGCUauACU---UCCG- -5' |
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1313 | 3' | -55.3 | NC_001331.1 | + | 4280 | 0.76 | 0.028984 |
Target: 5'- cGCCGccGGCCGGGagcgcaaggcaugaGCGAUAggccGAAGGCg -3' miRNA: 3'- -UGGCuaCCGGUCC--------------CGCUAUa---CUUCCG- -5' |
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1313 | 3' | -55.3 | NC_001331.1 | + | 4134 | 0.66 | 0.181186 |
Target: 5'- -aCGAUGGUCAGcGGCGAcuggccccuggccgGUGcgucugcgGAGGCg -3' miRNA: 3'- ugGCUACCGGUC-CCGCUa-------------UAC--------UUCCG- -5' |
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1313 | 3' | -55.3 | NC_001331.1 | + | 3539 | 0.75 | 0.032478 |
Target: 5'- uGCCGGUGGCCGuGGacuGGUA-GAGGGCg -3' miRNA: 3'- -UGGCUACCGGUcCCg--CUAUaCUUCCG- -5' |
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1313 | 3' | -55.3 | NC_001331.1 | + | 964 | 1.1 | 3.6e-05 |
Target: 5'- cACCGAUGGCCAGGGCGAUAUGAAGGCc -3' miRNA: 3'- -UGGCUACCGGUCCCGCUAUACUUCCG- -5' |
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1313 | 3' | -55.3 | NC_001331.1 | + | 915 | 0.66 | 0.184073 |
Target: 5'- uGCCGGccuucGCCGGGGUGAUcgacaccagcgcgGUGGAaucGGCg -3' miRNA: 3'- -UGGCUac---CGGUCCCGCUA-------------UACUU---CCG- -5' |
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1313 | 3' | -55.3 | NC_001331.1 | + | 352 | 0.66 | 0.167879 |
Target: 5'- gGCCGG-GGCUuGGGCGGUcgGcgccaucGGCu -3' miRNA: 3'- -UGGCUaCCGGuCCCGCUAuaCuu-----CCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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