Results 41 - 47 of 47 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13130 | 5' | -53.6 | NC_003401.1 | + | 26747 | 0.66 | 0.950853 |
Target: 5'- cGCG-GUgCGGCAUACGGuuuaacgugcAACUgCCGGu -3' miRNA: 3'- aCGCuCAgGCCGUAUGUU----------UUGG-GGCC- -5' |
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13130 | 5' | -53.6 | NC_003401.1 | + | 21005 | 0.66 | 0.954925 |
Target: 5'- gGCGuAG-CUGGCGauguuaaACGGGACCCCGa -3' miRNA: 3'- aCGC-UCaGGCCGUa------UGUUUUGGGGCc -5' |
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13130 | 5' | -53.6 | NC_003401.1 | + | 28144 | 0.66 | 0.96237 |
Target: 5'- gGCGAGgaggUCGGCAUACAcaaGGACgUCa- -3' miRNA: 3'- aCGCUCa---GGCCGUAUGU---UUUGgGGcc -5' |
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13130 | 5' | -53.6 | NC_003401.1 | + | 44704 | 0.66 | 0.96237 |
Target: 5'- -cCGGGUCUGGUGUuuGCGGuuAACCCCa- -3' miRNA: 3'- acGCUCAGGCCGUA--UGUU--UUGGGGcc -5' |
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13130 | 5' | -53.6 | NC_003401.1 | + | 123361 | 0.66 | 0.96237 |
Target: 5'- gGCG-GUuuGGCcgcgGCGcgGCCCCuGGa -3' miRNA: 3'- aCGCuCAggCCGua--UGUuuUGGGG-CC- -5' |
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13130 | 5' | -53.6 | NC_003401.1 | + | 20363 | 0.66 | 0.96375 |
Target: 5'- cGCGGGgCCGaGUGUAUGAAcgccaugguucgcggGCUCCGGg -3' miRNA: 3'- aCGCUCaGGC-CGUAUGUUU---------------UGGGGCC- -5' |
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13130 | 5' | -53.6 | NC_003401.1 | + | 132220 | 0.66 | 0.965752 |
Target: 5'- gGCGc--CCGGCcgGCGccGCCgCCGGg -3' miRNA: 3'- aCGCucaGGCCGuaUGUuuUGG-GGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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