Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13130 | 5' | -53.6 | NC_003401.1 | + | 132436 | 0.7 | 0.810388 |
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Target: 5'- cGCGGGcCCGGgGggcgcGCGGGggacccucGCCCCGGg -3' miRNA: 3'- aCGCUCaGGCCgUa----UGUUU--------UGGGGCC- -5' |
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| 13130 | 5' | -53.6 | NC_003401.1 | + | 132464 | 0.68 | 0.888569 |
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Target: 5'- cGcCGGGcCCGGCcgACucccgggagcGCCCCGGu -3' miRNA: 3'- aC-GCUCaGGCCGuaUGuuu-------UGGGGCC- -5' |
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| 13130 | 5' | -53.6 | NC_003401.1 | + | 132497 | 0.67 | 0.932146 |
|
Target: 5'- cGCGGGcccgggCCGGCGggGCGGAGggCCGGg -3' miRNA: 3'- aCGCUCa-----GGCCGUa-UGUUUUggGGCC- -5' |
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| 13130 | 5' | -53.6 | NC_003401.1 | + | 132610 | 0.68 | 0.896705 |
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Target: 5'- gGcCGGGcCCGGCGgaGCGGcacccgggggcGGCCCCGGc -3' miRNA: 3'- aC-GCUCaGGCCGUa-UGUU-----------UUGGGGCC- -5' |
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| 13130 | 5' | -53.6 | NC_003401.1 | + | 132690 | 0.74 | 0.599506 |
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Target: 5'- gGCGGGgcCCGGCGcgggGCGcgaccgaGGGCCCCGGg -3' miRNA: 3'- aCGCUCa-GGCCGUa---UGU-------UUUGGGGCC- -5' |
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| 13130 | 5' | -53.6 | NC_003401.1 | + | 132723 | 0.68 | 0.892 |
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Target: 5'- cGCGGccGcCCGGCAUcgggagccgcgcgccGCGgcuugugGGGCCCCGGg -3' miRNA: 3'- aCGCU--CaGGCCGUA---------------UGU-------UUUGGGGCC- -5' |
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| 13130 | 5' | -53.6 | NC_003401.1 | + | 133700 | 0.69 | 0.868289 |
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Target: 5'- gGCG-GUCCgGGCGggGgAGAGgCCCGGg -3' miRNA: 3'- aCGCuCAGG-CCGUa-UgUUUUgGGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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