Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13134 | 5' | -54.8 | NC_003401.1 | + | 121636 | 0.72 | 0.673495 |
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Target: 5'- cGGuGCAgGUGA-GUGgGGGCCGGGGu -3' miRNA: 3'- cCCuUGUaCGCUaUACgCCCGGUCCC- -5' |
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| 13134 | 5' | -54.8 | NC_003401.1 | + | 121137 | 0.72 | 0.632257 |
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Target: 5'- uGGGA--AUGgGGUAUcgcgGCGGGCgCGGGGg -3' miRNA: 3'- -CCCUugUACgCUAUA----CGCCCG-GUCCC- -5' |
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| 13134 | 5' | -54.8 | NC_003401.1 | + | 69560 | 0.72 | 0.632257 |
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Target: 5'- cGGGGACAUuggGCGGUAacGCGGGCUGcGGa -3' miRNA: 3'- -CCCUUGUA---CGCUAUa-CGCCCGGUcCC- -5' |
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| 13134 | 5' | -54.8 | NC_003401.1 | + | 120411 | 0.73 | 0.591013 |
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Target: 5'- uGGGGACGUG-GA--UGCGGucuguuGCCAGGGc -3' miRNA: 3'- -CCCUUGUACgCUauACGCC------CGGUCCC- -5' |
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| 13134 | 5' | -54.8 | NC_003401.1 | + | 132510 | 0.75 | 0.500616 |
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Target: 5'- cGGGGGCG-GCGGcc-GCGGGCCcGGGc -3' miRNA: 3'- -CCCUUGUaCGCUauaCGCCCGGuCCC- -5' |
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| 13134 | 5' | -54.8 | NC_003401.1 | + | 121678 | 0.77 | 0.399404 |
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Target: 5'- uGGGGGCGaaUGCGA--UGCGGgggauGCCGGGGa -3' miRNA: 3'- -CCCUUGU--ACGCUauACGCC-----CGGUCCC- -5' |
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| 13134 | 5' | -54.8 | NC_003401.1 | + | 69462 | 1.13 | 0.001801 |
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Target: 5'- gGGGAACAUGCGAUAUGCGGGCCAGGGg -3' miRNA: 3'- -CCCUUGUACGCUAUACGCCCGGUCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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