Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1314 | 5' | -56.7 | NC_001331.1 | + | 5438 | 0.67 | 0.149158 |
Target: 5'- cGCuCGAUCa-GCGGAUCgCAcUCGACCUg -3' miRNA: 3'- -UG-GCUAGggUGCCUAG-GUuGGCUGGG- -5' |
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1314 | 5' | -56.7 | NC_001331.1 | + | 4986 | 0.7 | 0.078447 |
Target: 5'- cGCuCGAUCCCACuGAUuuG-CCGuACCCg -3' miRNA: 3'- -UG-GCUAGGGUGcCUAggUuGGC-UGGG- -5' |
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1314 | 5' | -56.7 | NC_001331.1 | + | 3956 | 0.71 | 0.0711 |
Target: 5'- aGCCGAUCaguugCCAgcUGGugaguUCCAgguGCCGGCCCg -3' miRNA: 3'- -UGGCUAG-----GGU--GCCu----AGGU---UGGCUGGG- -5' |
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1314 | 5' | -56.7 | NC_001331.1 | + | 3901 | 0.66 | 0.168998 |
Target: 5'- --gGAUCCCACGGG-CCGGC--ACCUg -3' miRNA: 3'- uggCUAGGGUGCCUaGGUUGgcUGGG- -5' |
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1314 | 5' | -56.7 | NC_001331.1 | + | 3301 | 0.66 | 0.168998 |
Target: 5'- uGCCGGUgCC--GGGUCCAagcgucgaGCCaGCCCa -3' miRNA: 3'- -UGGCUAgGGugCCUAGGU--------UGGcUGGG- -5' |
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1314 | 5' | -56.7 | NC_001331.1 | + | 3228 | 0.73 | 0.044736 |
Target: 5'- gACCGGaagcggCCCACGcGG-CCGACCGcCCCa -3' miRNA: 3'- -UGGCUa-----GGGUGC-CUaGGUUGGCuGGG- -5' |
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1314 | 5' | -56.7 | NC_001331.1 | + | 2939 | 0.66 | 0.173777 |
Target: 5'- aACC--UCCgGCGcGAUCCAggaugacGCCGugCCc -3' miRNA: 3'- -UGGcuAGGgUGC-CUAGGU-------UGGCugGG- -5' |
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1314 | 5' | -56.7 | NC_001331.1 | + | 1978 | 1.04 | 0.000122 |
Target: 5'- gACCGAUCCCACGGAUCC-ACCGACCCg -3' miRNA: 3'- -UGGCUAGGGUGCCUAGGuUGGCUGGG- -5' |
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1314 | 5' | -56.7 | NC_001331.1 | + | 1900 | 0.71 | 0.068802 |
Target: 5'- cACCGAUCCCuCcGAcCCGAaCGACCCc -3' miRNA: 3'- -UGGCUAGGGuGcCUaGGUUgGCUGGG- -5' |
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1314 | 5' | -56.7 | NC_001331.1 | + | 1006 | 0.67 | 0.135694 |
Target: 5'- gUCGGcgCCCugGuGAUCCuggccgucGCCGGCCUg -3' miRNA: 3'- uGGCUa-GGGugC-CUAGGu-------UGGCUGGG- -5' |
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1314 | 5' | -56.7 | NC_001331.1 | + | 320 | 0.66 | 0.158799 |
Target: 5'- uGCCG--CCCACGG--CCAGCaGAUCCa -3' miRNA: 3'- -UGGCuaGGGUGCCuaGGUUGgCUGGG- -5' |
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1314 | 5' | -56.7 | NC_001331.1 | + | 300 | 0.68 | 0.118718 |
Target: 5'- gGCCGugggcggcaagCCgAUGGcgCCGACCG-CCCa -3' miRNA: 3'- -UGGCua---------GGgUGCCuaGGUUGGCuGGG- -5' |
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1314 | 5' | -56.7 | NC_001331.1 | + | 209 | 0.72 | 0.054065 |
Target: 5'- aGCCGGUgCUcugcaaguucgccaGCGuGGUCCGcCCGACCCa -3' miRNA: 3'- -UGGCUAgGG--------------UGC-CUAGGUuGGCUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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