Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13141 | 5' | -51.8 | NC_003401.1 | + | 27773 | 0.66 | 0.972271 |
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Target: 5'- uGGGgCGUGGAGAUGG-GugUCcGUUc -3' miRNA: 3'- cUUCgGCACCUCUAUCuCugAGaCAA- -5' |
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| 13141 | 5' | -51.8 | NC_003401.1 | + | 93029 | 0.66 | 0.969238 |
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Target: 5'- aGAGGCCGUGGAuguaugcgacgGAgGGGGAUUCg--- -3' miRNA: 3'- -CUUCGGCACCU-----------CUaUCUCUGAGacaa -5' |
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| 13141 | 5' | -51.8 | NC_003401.1 | + | 52185 | 0.67 | 0.950505 |
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Target: 5'- cGAGCUGUacGGAGAacUGGAGAUUCUa-- -3' miRNA: 3'- cUUCGGCA--CCUCU--AUCUCUGAGAcaa -5' |
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| 13141 | 5' | -51.8 | NC_003401.1 | + | 87642 | 0.68 | 0.936219 |
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Target: 5'- gGAGGCC-UGGGGuucUGGGGGCUCgGUa -3' miRNA: 3'- -CUUCGGcACCUCu--AUCUCUGAGaCAa -5' |
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| 13141 | 5' | -51.8 | NC_003401.1 | + | 19498 | 0.7 | 0.847196 |
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Target: 5'- cGAA-CCG-GGAGAUAGAGAUUUUGg- -3' miRNA: 3'- -CUUcGGCaCCUCUAUCUCUGAGACaa -5' |
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| 13141 | 5' | -51.8 | NC_003401.1 | + | 93157 | 1.05 | 0.008909 |
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Target: 5'- cGAAGCCGUGGAGAUAGAGACUCUGUUu -3' miRNA: 3'- -CUUCGGCACCUCUAUCUCUGAGACAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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