Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13143 | 3' | -51.3 | NC_003401.1 | + | 40669 | 0.66 | 0.989633 |
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Target: 5'- -uGUgGCCGACGuG-GAAUAcuGAGGAc -3' miRNA: 3'- cuCAgCGGCUGC-CaCUUAUauCUCCU- -5' |
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| 13143 | 3' | -51.3 | NC_003401.1 | + | 125305 | 0.66 | 0.986599 |
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Target: 5'- ---aUGCUGAUGGUGGucugggggAUGUGGAGGu -3' miRNA: 3'- cucaGCGGCUGCCACU--------UAUAUCUCCu -5' |
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| 13143 | 3' | -51.3 | NC_003401.1 | + | 97881 | 0.68 | 0.960439 |
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Target: 5'- cAGuUCGUCGACaGGUGGGaAUAGGGGc -3' miRNA: 3'- cUC-AGCGGCUG-CCACUUaUAUCUCCu -5' |
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| 13143 | 3' | -51.3 | NC_003401.1 | + | 19382 | 0.68 | 0.951304 |
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Target: 5'- aGGGUCGCCGucacggggccguCGGUGAcgAUuguuuccGGGGGGc -3' miRNA: 3'- -CUCAGCGGCu-----------GCCACUuaUA-------UCUCCU- -5' |
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| 13143 | 3' | -51.3 | NC_003401.1 | + | 36443 | 0.69 | 0.933835 |
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Target: 5'- uGGUCGCUGGCGGUugcuu--GAGGAu -3' miRNA: 3'- cUCAGCGGCUGCCAcuuauauCUCCU- -5' |
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| 13143 | 3' | -51.3 | NC_003401.1 | + | 118257 | 0.73 | 0.772347 |
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Target: 5'- gGGGUCGCCGGCGGgucaccgaaacaUGuacucAUGGGGGAa -3' miRNA: 3'- -CUCAGCGGCUGCC------------ACuua--UAUCUCCU- -5' |
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| 13143 | 3' | -51.3 | NC_003401.1 | + | 118612 | 0.76 | 0.638088 |
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Target: 5'- gGGGUUGCCGGuCGGUGAAcg-GGAGGc -3' miRNA: 3'- -CUCAGCGGCU-GCCACUUauaUCUCCu -5' |
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| 13143 | 3' | -51.3 | NC_003401.1 | + | 101195 | 1.09 | 0.00682 |
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Target: 5'- gGAGUCGCCGACGGUGAAUAUAGAGGAg -3' miRNA: 3'- -CUCAGCGGCUGCCACUUAUAUCUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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