Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13146 | 3' | -53.6 | NC_003401.1 | + | 41359 | 0.66 | 0.934011 |
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Target: 5'- aAAUCGCGGGccacGAGGUGgugggGCGCAggcccgCGUc -3' miRNA: 3'- -UUGGCGCCU----UUCCACa----CGUGUa-----GCAu -5' |
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| 13146 | 3' | -53.6 | NC_003401.1 | + | 8241 | 0.67 | 0.911099 |
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Target: 5'- cACCGCGGGauccGAGGcgGUGCA-GUCGg- -3' miRNA: 3'- uUGGCGCCU----UUCCa-CACGUgUAGCau -5' |
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| 13146 | 3' | -53.6 | NC_003401.1 | + | 8055 | 0.67 | 0.897417 |
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Target: 5'- cGACCGCGGGGuccucacGGG-GUGCGaacugaaaAUCGUAc -3' miRNA: 3'- -UUGGCGCCUU-------UCCaCACGUg-------UAGCAU- -5' |
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| 13146 | 3' | -53.6 | NC_003401.1 | + | 118323 | 0.68 | 0.875959 |
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Target: 5'- -cCCGCGGGucacugaAAGaUGUGCACAUgGUGc -3' miRNA: 3'- uuGGCGCCU-------UUCcACACGUGUAgCAU- -5' |
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| 13146 | 3' | -53.6 | NC_003401.1 | + | 14443 | 0.69 | 0.818817 |
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Target: 5'- cGCgGCGGAcAGGUGUGC-CGagGUAc -3' miRNA: 3'- uUGgCGCCUuUCCACACGuGUagCAU- -5' |
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| 13146 | 3' | -53.6 | NC_003401.1 | + | 72990 | 0.69 | 0.79107 |
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Target: 5'- gAGCCGCGGAGGGGUuuGUaGuCACAggaggCGUGc -3' miRNA: 3'- -UUGGCGCCUUUCCA--CA-C-GUGUa----GCAU- -5' |
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| 13146 | 3' | -53.6 | NC_003401.1 | + | 26541 | 0.7 | 0.765878 |
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Target: 5'- uGCCGCGGuuuGGGUuguacauacacgcggGUGCGCAUUGg- -3' miRNA: 3'- uUGGCGCCuu-UCCA---------------CACGUGUAGCau -5' |
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| 13146 | 3' | -53.6 | NC_003401.1 | + | 113273 | 1.05 | 0.006276 |
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Target: 5'- gAACCGCGGAAAGGUGUGCACAUCGUAa -3' miRNA: 3'- -UUGGCGCCUUUCCACACGUGUAGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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