Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13147 | 5' | -45.3 | NC_003401.1 | + | 105174 | 0.66 | 0.999983 |
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Target: 5'- cGUgGGCGGUGgugUGUUCGU--ACGUGu -3' miRNA: 3'- -UAgUUGUCACa--ACAAGCAcuUGCAUc -5' |
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| 13147 | 5' | -45.3 | NC_003401.1 | + | 118605 | 0.66 | 0.999976 |
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Target: 5'- cAUCGACGGgGUUGccggUCGgUGAACGggAGg -3' miRNA: 3'- -UAGUUGUCaCAACa---AGC-ACUUGCa-UC- -5' |
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| 13147 | 5' | -45.3 | NC_003401.1 | + | 106693 | 0.68 | 0.999695 |
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Target: 5'- gGUUGACGGUG-UGUUaCGUGAcGCGUGu -3' miRNA: 3'- -UAGUUGUCACaACAA-GCACU-UGCAUc -5' |
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| 13147 | 5' | -45.3 | NC_003401.1 | + | 67819 | 0.69 | 0.999382 |
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Target: 5'- gAUCGugAG-GggGUaacaauUCGUGGGCGUGGg -3' miRNA: 3'- -UAGUugUCaCaaCA------AGCACUUGCAUC- -5' |
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| 13147 | 5' | -45.3 | NC_003401.1 | + | 113254 | 0.69 | 0.998823 |
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Target: 5'- -aCAAUAGUGUUGUUgGUGAcCGc-- -3' miRNA: 3'- uaGUUGUCACAACAAgCACUuGCauc -5' |
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| 13147 | 5' | -45.3 | NC_003401.1 | + | 113380 | 1.09 | 0.027752 |
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Target: 5'- gAUCAACAGUGUUGUUCGUGAACGUAGa -3' miRNA: 3'- -UAGUUGUCACAACAAGCACUUGCAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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