Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13148 | 5' | -51.7 | NC_003401.1 | + | 62350 | 0.66 | 0.980011 |
Target: 5'- -cUGGGuCGaacGUUCagUGGAUUCCGUg -3' miRNA: 3'- uaGCCC-GCa--CAAGaaACCUAAGGCAg -5' |
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13148 | 5' | -51.7 | NC_003401.1 | + | 29308 | 0.66 | 0.977638 |
Target: 5'- cUCGGGCGUaagGUUCcggUGGAgUUCUGg- -3' miRNA: 3'- uAGCCCGCA---CAAGaa-ACCU-AAGGCag -5' |
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13148 | 5' | -51.7 | NC_003401.1 | + | 89191 | 0.68 | 0.941629 |
Target: 5'- uUCGGGCG-GcUCggUGGAUUuuGUa -3' miRNA: 3'- uAGCCCGCaCaAGaaACCUAAggCAg -5' |
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13148 | 5' | -51.7 | NC_003401.1 | + | 113420 | 0.76 | 0.598817 |
Target: 5'- -aCGGGCacGUG-UCUUUGGGUUCCGg- -3' miRNA: 3'- uaGCCCG--CACaAGAAACCUAAGGCag -5' |
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13148 | 5' | -51.7 | NC_003401.1 | + | 113544 | 1.08 | 0.006858 |
Target: 5'- gAUCGGGCGUGUUCUUUGGAUUCCGUCu -3' miRNA: 3'- -UAGCCCGCACAAGAAACCUAAGGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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