Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13149 | 5' | -41.1 | NC_003401.1 | + | 124499 | 0.66 | 1 |
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Target: 5'- gUGACGCAGGGCCg-------UCCu- -3' miRNA: 3'- aAUUGCGUUUCGGaauuaaaaAGGua -5' |
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| 13149 | 5' | -41.1 | NC_003401.1 | + | 105482 | 0.69 | 0.999999 |
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Target: 5'- -cAACGCAAGGCCagGGUggcggCCGUc -3' miRNA: 3'- aaUUGCGUUUCGGaaUUAaaaa-GGUA- -5' |
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| 13149 | 5' | -41.1 | NC_003401.1 | + | 45821 | 0.69 | 0.999998 |
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Target: 5'- -cAACGUAuuGCCUUGAUUUgaUCUAUu -3' miRNA: 3'- aaUUGCGUuuCGGAAUUAAAa-AGGUA- -5' |
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| 13149 | 5' | -41.1 | NC_003401.1 | + | 104528 | 0.7 | 0.999985 |
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Target: 5'- gUGGCGCGAAGCaggGAUUcuaugcUUUCCAg -3' miRNA: 3'- aAUUGCGUUUCGgaaUUAA------AAAGGUa -5' |
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| 13149 | 5' | -41.1 | NC_003401.1 | + | 74298 | 0.7 | 0.999978 |
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Target: 5'- -gGACGCGAucgucguAGCC--AGUUUUUCCAUu -3' miRNA: 3'- aaUUGCGUU-------UCGGaaUUAAAAAGGUA- -5' |
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| 13149 | 5' | -41.1 | NC_003401.1 | + | 10513 | 0.73 | 0.999334 |
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Target: 5'- -aAACGCAcuGAGCCggGAUUgcUCCGUg -3' miRNA: 3'- aaUUGCGU--UUCGGaaUUAAaaAGGUA- -5' |
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| 13149 | 5' | -41.1 | NC_003401.1 | + | 91485 | 0.75 | 0.996474 |
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Target: 5'- --cGCGCGAGGCguCUUGGUUUUUCCu- -3' miRNA: 3'- aauUGCGUUUCG--GAAUUAAAAAGGua -5' |
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| 13149 | 5' | -41.1 | NC_003401.1 | + | 114435 | 0.85 | 0.787669 |
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Target: 5'- cUAACGCAAagGGCaCUUAAUUUUUCCAg -3' miRNA: 3'- aAUUGCGUU--UCG-GAAUUAAAAAGGUa -5' |
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| 13149 | 5' | -41.1 | NC_003401.1 | + | 114562 | 1.04 | 0.117029 |
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Target: 5'- uUUAACGCAAAGCCUUAAUUUUUCCAUu -3' miRNA: 3'- -AAUUGCGUUUCGGAAUUAAAAAGGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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