miRNA: 3'- acugCGGCACGg---GCGCGACUU-----------UCUGC- -5'
miRNA: 3'- aCUGcGGC--ACGGGCG---CGACUuuCugC- -5'
miRNA: 3'- -ACUGCGGC-ACG------GGCGCGACUuuCuGC- -5'
miRNA: 3'- acUGCGG----CACGGGCGCGacUUUCUGc -5'
miRNA: 3'- -ACUGCGGCACGGGCGCGACUUUCUGC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1315 | 3' | -57.4 | NC_001331.1 | + | 4873 | 0.66 | 0.139701 |
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Target: 5'- ---aGCUGUGCauauCGCGCUGGAugugcagggcuGGACGc -3' miRNA: 3'- acugCGGCACGg---GCGCGACUU-----------UCUGC- -5' |
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| 1315 | 3' | -57.4 | NC_001331.1 | + | 1921 | 0.67 | 0.115252 |
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Target: 5'- cGACcCCGgcUGCCCGCccgGCUGGucGugGu -3' miRNA: 3'- aCUGcGGC--ACGGGCG---CGACUuuCugC- -5' |
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| 1315 | 3' | -57.4 | NC_001331.1 | + | 2476 | 0.68 | 0.083191 |
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Target: 5'- gUGGCGCUGcUGCugauggCCGCGCUGAucGcCGc -3' miRNA: 3'- -ACUGCGGC-ACG------GGCGCGACUuuCuGC- -5' |
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| 1315 | 3' | -57.4 | NC_001331.1 | + | 6760 | 0.7 | 0.06177 |
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Target: 5'- -cACGCCcuuuGUGCCCGUGCgcGAAGACu -3' miRNA: 3'- acUGCGG----CACGGGCGCGacUUUCUGc -5' |
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| 1315 | 3' | -57.4 | NC_001331.1 | + | 2959 | 1.1 | 2.6e-05 |
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Target: 5'- aUGACGCCGUGCCCGCGCUGAAAGACGg -3' miRNA: 3'- -ACUGCGGCACGGGCGCGACUUUCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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