Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13150 | 5' | -49.3 | NC_003401.1 | + | 69781 | 0.66 | 0.995408 |
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Target: 5'- cUUAGCGGGGaccgUUGCGGCgguuucggcgaaGGGCGgaGAg -3' miRNA: 3'- -AAUUGUCCCa---AACGCUG------------UCUGUgaCU- -5' |
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| 13150 | 5' | -49.3 | NC_003401.1 | + | 18451 | 0.66 | 0.992717 |
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Target: 5'- -gGGCAGGGcgcgGCGGCGGcCACa-- -3' miRNA: 3'- aaUUGUCCCaaa-CGCUGUCuGUGacu -5' |
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| 13150 | 5' | -49.3 | NC_003401.1 | + | 111525 | 0.66 | 0.992717 |
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Target: 5'- -cGGCGuccGGGUgUGCGGcCAGACACUc- -3' miRNA: 3'- aaUUGU---CCCAaACGCU-GUCUGUGAcu -5' |
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| 13150 | 5' | -49.3 | NC_003401.1 | + | 117892 | 0.67 | 0.985584 |
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Target: 5'- --cGCGGGGguucgGCGGCGGugGCa-- -3' miRNA: 3'- aauUGUCCCaaa--CGCUGUCugUGacu -5' |
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| 13150 | 5' | -49.3 | NC_003401.1 | + | 121140 | 0.7 | 0.943603 |
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Target: 5'- -gAAUGGGGUaucGCGGCGGGCGCgGGg -3' miRNA: 3'- aaUUGUCCCAaa-CGCUGUCUGUGaCU- -5' |
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| 13150 | 5' | -49.3 | NC_003401.1 | + | 114743 | 1.07 | 0.012091 |
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Target: 5'- uUUAACAGGGUUUGCGACAGACACUGAu -3' miRNA: 3'- -AAUUGUCCCAAACGCUGUCUGUGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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