Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13154 | 3' | -53.1 | NC_003401.1 | + | 23757 | 0.66 | 0.95785 |
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Target: 5'- aGUUGGUCAGUUguCCcGUCAAUCAc -3' miRNA: 3'- gUAACCGGUCAGgcGGaCAGUUAGUc -5' |
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| 13154 | 3' | -53.1 | NC_003401.1 | + | 21168 | 0.66 | 0.953876 |
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Target: 5'- --aUGcGCCgaucguGGggCCGCCUGUUAAUUAGa -3' miRNA: 3'- guaAC-CGG------UCa-GGCGGACAGUUAGUC- -5' |
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| 13154 | 3' | -53.1 | NC_003401.1 | + | 88741 | 0.66 | 0.945189 |
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Target: 5'- gGUUGGCCAGcgcuauaCGCCa--CAGUCAGa -3' miRNA: 3'- gUAACCGGUCag-----GCGGacaGUUAGUC- -5' |
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| 13154 | 3' | -53.1 | NC_003401.1 | + | 120289 | 0.67 | 0.919025 |
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Target: 5'- ---aGGCUGGgggCCGCCUGUCuauuuUUAGc -3' miRNA: 3'- guaaCCGGUCa--GGCGGACAGuu---AGUC- -5' |
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| 13154 | 3' | -53.1 | NC_003401.1 | + | 127244 | 0.69 | 0.847931 |
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Target: 5'- ---gGGCCuauUCCGCCUggccuccgugGUCGAUCAGc -3' miRNA: 3'- guaaCCGGuc-AGGCGGA----------CAGUUAGUC- -5' |
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| 13154 | 3' | -53.1 | NC_003401.1 | + | 69145 | 0.71 | 0.756154 |
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Target: 5'- -----cCCAGUCUGCCUcaGUCAGUCAGc -3' miRNA: 3'- guaaccGGUCAGGCGGA--CAGUUAGUC- -5' |
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| 13154 | 3' | -53.1 | NC_003401.1 | + | 123191 | 0.72 | 0.705232 |
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Target: 5'- ---aGGCCAGUcCCGCaCcGUCGGUCAa -3' miRNA: 3'- guaaCCGGUCA-GGCG-GaCAGUUAGUc -5' |
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| 13154 | 3' | -53.1 | NC_003401.1 | + | 117797 | 0.74 | 0.599605 |
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Target: 5'- cCAUUGGCCGG-CCaaGCC-GUCAAUCAa -3' miRNA: 3'- -GUAACCGGUCaGG--CGGaCAGUUAGUc -5' |
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| 13154 | 3' | -53.1 | NC_003401.1 | + | 117702 | 0.8 | 0.319577 |
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Target: 5'- gAUUGGCCGG-CCGuUCUGUCAAUCAa -3' miRNA: 3'- gUAACCGGUCaGGC-GGACAGUUAGUc -5' |
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| 13154 | 3' | -53.1 | NC_003401.1 | + | 117663 | 1.1 | 0.003793 |
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Target: 5'- cCAUUGGCCAGUCCGCCUGUCAAUCAGg -3' miRNA: 3'- -GUAACCGGUCAGGCGGACAGUUAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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