Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13156 | 3' | -51.2 | NC_003401.1 | + | 67999 | 0.66 | 0.986366 |
Target: 5'- -----cAUGUUGCUCcGACACUGCg- -3' miRNA: 3'- cucuuaUACAACGGGaCUGUGGCGac -5' |
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13156 | 3' | -51.2 | NC_003401.1 | + | 30161 | 0.66 | 0.980417 |
Target: 5'- uGGGAUAUGgccuUUGCCUccaGCGCCGCUa -3' miRNA: 3'- cUCUUAUAC----AACGGGac-UGUGGCGAc -5' |
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13156 | 3' | -51.2 | NC_003401.1 | + | 31695 | 0.66 | 0.975494 |
Target: 5'- cGGAAUcugucaguugGUGUaaGCCCUGcCAUCGCUGu -3' miRNA: 3'- cUCUUA----------UACAa-CGGGACuGUGGCGAC- -5' |
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13156 | 3' | -51.2 | NC_003401.1 | + | 42926 | 0.67 | 0.966498 |
Target: 5'- uGAGAcccgGUAUGUUGUCCgagugGGCAcccCCGCa- -3' miRNA: 3'- -CUCU----UAUACAACGGGa----CUGU---GGCGac -5' |
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13156 | 3' | -51.2 | NC_003401.1 | + | 56222 | 0.67 | 0.95934 |
Target: 5'- cGGGAAUGUGgcgGUCagUGGCacuACCGCUGa -3' miRNA: 3'- -CUCUUAUACaa-CGGg-ACUG---UGGCGAC- -5' |
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13156 | 3' | -51.2 | NC_003401.1 | + | 118416 | 0.87 | 0.160639 |
Target: 5'- gGAGAAUAUGUU-CCgCUGACACCGCUGc -3' miRNA: 3'- -CUCUUAUACAAcGG-GACUGUGGCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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