Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13159 | 5' | -56.2 | NC_003401.1 | + | 95201 | 0.66 | 0.852276 |
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Target: 5'- cAGAugGCCAGUGGCaccuGUUUGCgCcagAGGUGGc -3' miRNA: 3'- -UCU--UGGUCACCG----CGAACG-G---UCCACUu -5' |
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| 13159 | 5' | -56.2 | NC_003401.1 | + | 129243 | 0.66 | 0.84407 |
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Target: 5'- uAGAugACCuccuGcUGGCGCgugGCUGGGUGAc -3' miRNA: 3'- -UCU--UGGu---C-ACCGCGaa-CGGUCCACUu -5' |
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| 13159 | 5' | -56.2 | NC_003401.1 | + | 20529 | 0.66 | 0.84407 |
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Target: 5'- cAGGACCAGggGGCGCcUGUCugaaGUGGGg -3' miRNA: 3'- -UCUUGGUCa-CCGCGaACGGuc--CACUU- -5' |
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| 13159 | 5' | -56.2 | NC_003401.1 | + | 125066 | 0.67 | 0.781371 |
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Target: 5'- gGGAgGCCAGgUGGCGCccGUCcGGUGAc -3' miRNA: 3'- -UCU-UGGUC-ACCGCGaaCGGuCCACUu -5' |
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| 13159 | 5' | -56.2 | NC_003401.1 | + | 7426 | 0.67 | 0.77177 |
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Target: 5'- uGGGGCgAGaaagacgcGGCGUagGCCAGGUGAu -3' miRNA: 3'- -UCUUGgUCa-------CCGCGaaCGGUCCACUu -5' |
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| 13159 | 5' | -56.2 | NC_003401.1 | + | 80732 | 0.68 | 0.720959 |
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Target: 5'- uGAACCGGUagccagcugacugGGCGCUgagggaauuaguUGCCgcAGGUGGGa -3' miRNA: 3'- uCUUGGUCA-------------CCGCGA------------ACGG--UCCACUU- -5' |
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| 13159 | 5' | -56.2 | NC_003401.1 | + | 64510 | 0.69 | 0.691041 |
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Target: 5'- gAGAACCGGUugccgcgugGGCGgUaGCCAGGgGAGa -3' miRNA: 3'- -UCUUGGUCA---------CCGCgAaCGGUCCaCUU- -5' |
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| 13159 | 5' | -56.2 | NC_003401.1 | + | 27348 | 0.69 | 0.680611 |
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Target: 5'- uGGcGCUAGUGGCGCUguuUGCCaucaggcgauAGGUGu- -3' miRNA: 3'- -UCuUGGUCACCGCGA---ACGG----------UCCACuu -5' |
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| 13159 | 5' | -56.2 | NC_003401.1 | + | 120853 | 1.06 | 0.002764 |
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Target: 5'- aAGAACCAGUGGCGCUUGCCAGGUGAAa -3' miRNA: 3'- -UCUUGGUCACCGCGAACGGUCCACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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