Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13160 | 3' | -59.2 | NC_003401.1 | + | 21908 | 0.7 | 0.535319 |
Target: 5'- aGCACCUcGGUuguaauuaugauuGUCCUGgugCAUCUCGGUa -3' miRNA: 3'- -CGUGGA-CCGu------------CAGGGCa--GUAGGGCCG- -5' |
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13160 | 3' | -59.2 | NC_003401.1 | + | 132592 | 0.7 | 0.519851 |
Target: 5'- gGCACCcgggGGCGGcCCCGgccccccgCGggaccCCCGGCc -3' miRNA: 3'- -CGUGGa---CCGUCaGGGCa-------GUa----GGGCCG- -5' |
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13160 | 3' | -59.2 | NC_003401.1 | + | 64229 | 0.72 | 0.410671 |
Target: 5'- cGUACCcgGGCGGUaccggcaacCCCGUaCGggaCCCGGCg -3' miRNA: 3'- -CGUGGa-CCGUCA---------GGGCA-GUa--GGGCCG- -5' |
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13160 | 3' | -59.2 | NC_003401.1 | + | 121052 | 1.13 | 0.000736 |
Target: 5'- cGCACCUGGCAGUCCCGUCAUCCCGGCu -3' miRNA: 3'- -CGUGGACCGUCAGGGCAGUAGGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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