Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13161 | 3' | -62.4 | NC_003401.1 | + | 117890 | 0.66 | 0.517488 |
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Target: 5'- ---cGGGGGuucGGCGGCGGUGGCAc -3' miRNA: 3'- uucuCCCCCuccUCGCCGUCGUCGUu -5' |
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| 13161 | 3' | -62.4 | NC_003401.1 | + | 121477 | 0.66 | 0.507748 |
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Target: 5'- cGGAGGGGGcgAGGAcGuCGGCGuuGGCGAu -3' miRNA: 3'- uUCUCCCCC--UCCU-C-GCCGUcgUCGUU- -5' |
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| 13161 | 3' | -62.4 | NC_003401.1 | + | 118901 | 0.66 | 0.495203 |
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Target: 5'- gGGGucguuGGGGGAGGAagccgcgccgccaaGCGGC-GCGGCc- -3' miRNA: 3'- -UUCu----CCCCCUCCU--------------CGCCGuCGUCGuu -5' |
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| 13161 | 3' | -62.4 | NC_003401.1 | + | 132468 | 0.68 | 0.398099 |
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Target: 5'- cGGGAgccGGGGGAGGccGGCGGCgcccGGCGcGCGGg -3' miRNA: 3'- -UUCU---CCCCCUCC--UCGCCG----UCGU-CGUU- -5' |
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| 13161 | 3' | -62.4 | NC_003401.1 | + | 18453 | 0.68 | 0.398099 |
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Target: 5'- ---cGGGGcAGGGcGCGGCGGCGGCc- -3' miRNA: 3'- uucuCCCCcUCCU-CGCCGUCGUCGuu -5' |
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| 13161 | 3' | -62.4 | NC_003401.1 | + | 132519 | 0.69 | 0.357098 |
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Target: 5'- --cGGGGcGGccGGGGGCGGCGGCcGCGGg -3' miRNA: 3'- uucUCCC-CC--UCCUCGCCGUCGuCGUU- -5' |
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| 13161 | 3' | -62.4 | NC_003401.1 | + | 84034 | 0.7 | 0.291035 |
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Target: 5'- cGGAGGgaccaacguGGGcaaGGGGGCgGGCAGCGGCAu -3' miRNA: 3'- uUCUCC---------CCC---UCCUCG-CCGUCGUCGUu -5' |
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| 13161 | 3' | -62.4 | NC_003401.1 | + | 121923 | 0.72 | 0.202792 |
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Target: 5'- uGGAGcGGGGGGcGCGGguGCGGCGg -3' miRNA: 3'- uUCUCcCCCUCCuCGCCguCGUCGUu -5' |
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| 13161 | 3' | -62.4 | NC_003401.1 | + | 121345 | 1.03 | 0.001149 |
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Target: 5'- cAAGAGGGGGAGGAGCGGCAGCAGCAAc -3' miRNA: 3'- -UUCUCCCCCUCCUCGCCGUCGUCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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