Results 1 - 20 of 60 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13162 | 5' | -54.5 | NC_003401.1 | + | 103510 | 0.66 | 0.948221 |
Target: 5'- aCCAUaggccugCCCaUggUGCGCAACcuGGCGGa -3' miRNA: 3'- gGGUAg------GGG-AuuGUGCGUUGu-CCGCC- -5' |
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13162 | 5' | -54.5 | NC_003401.1 | + | 132280 | 0.66 | 0.948221 |
Target: 5'- cCCCGcgcgCCCCccgGGCccGCGCGcCGGGCGc -3' miRNA: 3'- -GGGUa---GGGGa--UUG--UGCGUuGUCCGCc -5' |
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13162 | 5' | -54.5 | NC_003401.1 | + | 64684 | 0.66 | 0.948221 |
Target: 5'- aUCCGucUCCCCUGGCuaccgcccACGCGGCAacCGGu -3' miRNA: 3'- -GGGU--AGGGGAUUG--------UGCGUUGUccGCC- -5' |
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13162 | 5' | -54.5 | NC_003401.1 | + | 69574 | 0.66 | 0.943809 |
Target: 5'- uUCCAUCCuCUUGGCG-GgGACAuugGGCGGu -3' miRNA: 3'- -GGGUAGG-GGAUUGUgCgUUGU---CCGCC- -5' |
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13162 | 5' | -54.5 | NC_003401.1 | + | 17978 | 0.66 | 0.943355 |
Target: 5'- gUCAUCCgaaaacggccucaCCUGuaACGCAACAGGCu- -3' miRNA: 3'- gGGUAGG-------------GGAUugUGCGUUGUCCGcc -5' |
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13162 | 5' | -54.5 | NC_003401.1 | + | 128436 | 0.66 | 0.939159 |
Target: 5'- gCCCA-CCUCUAAC-CGCAACguucuaucgAGGCc- -3' miRNA: 3'- -GGGUaGGGGAUUGuGCGUUG---------UCCGcc -5' |
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13162 | 5' | -54.5 | NC_003401.1 | + | 88249 | 0.66 | 0.939159 |
Target: 5'- gUgGUCgCUUUGGCGCcgGCAACAGGCGa -3' miRNA: 3'- gGgUAG-GGGAUUGUG--CGUUGUCCGCc -5' |
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13162 | 5' | -54.5 | NC_003401.1 | + | 47955 | 0.66 | 0.939159 |
Target: 5'- aCCCGUCCCC--ACACGUauaaagucuuaGACGuGCGu -3' miRNA: 3'- -GGGUAGGGGauUGUGCG-----------UUGUcCGCc -5' |
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13162 | 5' | -54.5 | NC_003401.1 | + | 13020 | 0.66 | 0.929137 |
Target: 5'- gCCAUCUCCgugacgGACuGCGUGGC-GGUGGa -3' miRNA: 3'- gGGUAGGGGa-----UUG-UGCGUUGuCCGCC- -5' |
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13162 | 5' | -54.5 | NC_003401.1 | + | 127540 | 0.66 | 0.929137 |
Target: 5'- gCCGUCCUCc--CGCGCGACAcGGagcCGGa -3' miRNA: 3'- gGGUAGGGGauuGUGCGUUGU-CC---GCC- -5' |
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13162 | 5' | -54.5 | NC_003401.1 | + | 104537 | 0.66 | 0.929137 |
Target: 5'- gCCGUCCUCgUGGCGCGaAGCAGGg-- -3' miRNA: 3'- gGGUAGGGG-AUUGUGCgUUGUCCgcc -5' |
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13162 | 5' | -54.5 | NC_003401.1 | + | 9356 | 0.67 | 0.923762 |
Target: 5'- aCCGaCCCCUAGCgccgGCGCccuACuGGcCGGg -3' miRNA: 3'- gGGUaGGGGAUUG----UGCGu--UGuCC-GCC- -5' |
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13162 | 5' | -54.5 | NC_003401.1 | + | 49071 | 0.67 | 0.923762 |
Target: 5'- gCC--CCCCgggAGCAC-CAGcCAGGCGGu -3' miRNA: 3'- gGGuaGGGGa--UUGUGcGUU-GUCCGCC- -5' |
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13162 | 5' | -54.5 | NC_003401.1 | + | 40349 | 0.67 | 0.918146 |
Target: 5'- uUUcgCCCCUGACaACGUAAUuGGCGu -3' miRNA: 3'- gGGuaGGGGAUUG-UGCGUUGuCCGCc -5' |
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13162 | 5' | -54.5 | NC_003401.1 | + | 22331 | 0.67 | 0.918146 |
Target: 5'- gUCGUCCCCggGAUugGCAcACAGcuuuuuaccGCGGc -3' miRNA: 3'- gGGUAGGGGa-UUGugCGU-UGUC---------CGCC- -5' |
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13162 | 5' | -54.5 | NC_003401.1 | + | 40286 | 0.67 | 0.918146 |
Target: 5'- aCCGUCCCa--ACGCGCAGCccaGCGu -3' miRNA: 3'- gGGUAGGGgauUGUGCGUUGuc-CGCc -5' |
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13162 | 5' | -54.5 | NC_003401.1 | + | 57485 | 0.67 | 0.918146 |
Target: 5'- aCCCGUUCgUUGGC-CGCuuUGGGUGGa -3' miRNA: 3'- -GGGUAGGgGAUUGuGCGuuGUCCGCC- -5' |
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13162 | 5' | -54.5 | NC_003401.1 | + | 47820 | 0.67 | 0.918146 |
Target: 5'- aCCCGUCgCaCUGACcCGCGACGGaucgcCGGu -3' miRNA: 3'- -GGGUAGgG-GAUUGuGCGUUGUCc----GCC- -5' |
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13162 | 5' | -54.5 | NC_003401.1 | + | 126221 | 0.67 | 0.90619 |
Target: 5'- aUCCAgCCCC-AGCACGuCGACgAGGUGc -3' miRNA: 3'- -GGGUaGGGGaUUGUGC-GUUG-UCCGCc -5' |
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13162 | 5' | -54.5 | NC_003401.1 | + | 132373 | 0.67 | 0.90619 |
Target: 5'- cCCCGgccgCCCCgcgcCGCGCGcCGGGCc- -3' miRNA: 3'- -GGGUa---GGGGauu-GUGCGUuGUCCGcc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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