Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13163 | 5' | -55 | NC_003401.1 | + | 121926 | 0.7 | 0.676778 |
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Target: 5'- aGCGGGgGGCGCgGGUGCgGCGGc--- -3' miRNA: 3'- -CGUCUgUCGCGgUCGCGgUGCUaauu -5' |
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| 13163 | 5' | -55 | NC_003401.1 | + | 122097 | 0.7 | 0.708008 |
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Target: 5'- gGCAcGGCcGCGCCAGCcGCCGCa----- -3' miRNA: 3'- -CGU-CUGuCGCGGUCG-CGGUGcuaauu -5' |
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| 13163 | 5' | -55 | NC_003401.1 | + | 122415 | 0.65 | 0.907722 |
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Target: 5'- uGCGGuuaACGGCGCuCuGCGCCACc----- -3' miRNA: 3'- -CGUC---UGUCGCG-GuCGCGGUGcuaauu -5' |
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| 13163 | 5' | -55 | NC_003401.1 | + | 124466 | 1.08 | 0.002872 |
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Target: 5'- gGCAGACAGCGCCAGCGCCACGAUUAAa -3' miRNA: 3'- -CGUCUGUCGCGGUCGCGGUGCUAAUU- -5' |
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| 13163 | 5' | -55 | NC_003401.1 | + | 126727 | 0.68 | 0.824043 |
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Target: 5'- cGCAGAUGGCGUaucucGCGCCACu----- -3' miRNA: 3'- -CGUCUGUCGCGgu---CGCGGUGcuaauu -5' |
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| 13163 | 5' | -55 | NC_003401.1 | + | 128469 | 0.7 | 0.676778 |
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Target: 5'- cGCGuGCAGCGCCAGCuCC-CGGUg-- -3' miRNA: 3'- -CGUcUGUCGCGGUCGcGGuGCUAauu -5' |
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| 13163 | 5' | -55 | NC_003401.1 | + | 132375 | 0.66 | 0.873084 |
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Target: 5'- gGCGG-CGGCGCCggccgGGCGCCucCGGg--- -3' miRNA: 3'- -CGUCuGUCGCGG-----UCGCGGu-GCUaauu -5' |
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| 13163 | 5' | -55 | NC_003401.1 | + | 132454 | 0.67 | 0.865446 |
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Target: 5'- gGCcGGCGGCGcCCGGCG-CGCGGg--- -3' miRNA: 3'- -CGuCUGUCGC-GGUCGCgGUGCUaauu -5' |
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| 13163 | 5' | -55 | NC_003401.1 | + | 132510 | 0.68 | 0.787532 |
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Target: 5'- --cGGCGGCGCC-GCGCCcCGAc--- -3' miRNA: 3'- cguCUGUCGCGGuCGCGGuGCUaauu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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