Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13163 | 5' | -55 | NC_003401.1 | + | 132510 | 0.68 | 0.787532 |
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Target: 5'- --cGGCGGCGCC-GCGCCcCGAc--- -3' miRNA: 3'- cguCUGUCGCGGuCGCGGuGCUaauu -5' |
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| 13163 | 5' | -55 | NC_003401.1 | + | 20551 | 0.68 | 0.824043 |
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Target: 5'- cGCGGGCGGCgGCCGGUcaacGCCAgGuccAUUAAa -3' miRNA: 3'- -CGUCUGUCG-CGGUCG----CGGUgC---UAAUU- -5' |
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| 13163 | 5' | -55 | NC_003401.1 | + | 46543 | 0.68 | 0.824043 |
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Target: 5'- uGCAGGCGGUaGCaCGGgcaCGCCACGAc--- -3' miRNA: 3'- -CGUCUGUCG-CG-GUC---GCGGUGCUaauu -5' |
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| 13163 | 5' | -55 | NC_003401.1 | + | 126727 | 0.68 | 0.824043 |
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Target: 5'- cGCAGAUGGCGUaucucGCGCCACu----- -3' miRNA: 3'- -CGUCUGUCGCGgu---CGCGGUGcuaauu -5' |
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| 13163 | 5' | -55 | NC_003401.1 | + | 72795 | 0.67 | 0.829276 |
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Target: 5'- uCAGGCGGCcCCugaggucacucguGCGCCACGGUUu- -3' miRNA: 3'- cGUCUGUCGcGGu------------CGCGGUGCUAAuu -5' |
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| 13163 | 5' | -55 | NC_003401.1 | + | 23618 | 0.67 | 0.841219 |
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Target: 5'- gGCAG-CAG-GCCaaaaacacgGGCGCCGCGAg--- -3' miRNA: 3'- -CGUCuGUCgCGG---------UCGCGGUGCUaauu -5' |
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| 13163 | 5' | -55 | NC_003401.1 | + | 22612 | 0.66 | 0.873084 |
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Target: 5'- uGCcacCAGCGCCAcGCucgccgugcucgGCCACGGUUGAc -3' miRNA: 3'- -CGucuGUCGCGGU-CG------------CGGUGCUAAUU- -5' |
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| 13163 | 5' | -55 | NC_003401.1 | + | 28349 | 0.66 | 0.880491 |
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Target: 5'- -gGGAgGGCGCUcaugguucgGGUGUCGCGGUUAc -3' miRNA: 3'- cgUCUgUCGCGG---------UCGCGGUGCUAAUu -5' |
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| 13163 | 5' | -55 | NC_003401.1 | + | 27597 | 0.75 | 0.388053 |
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Target: 5'- gGCGGGCAcGCGCCAGCGCgGgGGg--- -3' miRNA: 3'- -CGUCUGU-CGCGGUCGCGgUgCUaauu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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