Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13164 | 5' | -53.7 | NC_003401.1 | + | 23653 | 0.67 | 0.935595 |
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Target: 5'- cGGcagGUg-GCGCcGCGCUuuccCCCACGCc -3' miRNA: 3'- -CCua-CAagCGCGuCGUGAu---GGGUGCG- -5' |
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| 13164 | 5' | -53.7 | NC_003401.1 | + | 100755 | 0.67 | 0.940479 |
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Target: 5'- gGGAUGUcuUCGaUGguGCACgGCuCUAUGCc -3' miRNA: 3'- -CCUACA--AGC-GCguCGUGaUG-GGUGCG- -5' |
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| 13164 | 5' | -53.7 | NC_003401.1 | + | 45778 | 0.66 | 0.945116 |
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Target: 5'- --uUGuUUCGCGCAGUACgugugACCUcCGUc -3' miRNA: 3'- ccuAC-AAGCGCGUCGUGa----UGGGuGCG- -5' |
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| 13164 | 5' | -53.7 | NC_003401.1 | + | 11851 | 0.66 | 0.949511 |
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Target: 5'- ---aGUUCGCcggugGCcgaGGCGCUGCaCACGCg -3' miRNA: 3'- ccuaCAAGCG-----CG---UCGUGAUGgGUGCG- -5' |
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| 13164 | 5' | -53.7 | NC_003401.1 | + | 35496 | 0.66 | 0.95326 |
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Target: 5'- uGAUGUUCGUaaGCGGCAUcagcgcgguuacuUGCuuCCAUGCc -3' miRNA: 3'- cCUACAAGCG--CGUCGUG-------------AUG--GGUGCG- -5' |
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| 13164 | 5' | -53.7 | NC_003401.1 | + | 90905 | 0.66 | 0.953665 |
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Target: 5'- cGGAUGUgacCGCAGCGCUcugagugaACaaACGCc -3' miRNA: 3'- -CCUACAagcGCGUCGUGA--------UGggUGCG- -5' |
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| 13164 | 5' | -53.7 | NC_003401.1 | + | 85874 | 0.66 | 0.961266 |
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Target: 5'- cGGUGUgucCGCGCGugugcGCGCUccGCCUugaguuGCGCa -3' miRNA: 3'- cCUACAa--GCGCGU-----CGUGA--UGGG------UGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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