Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13167 | 3' | -51.9 | NC_003401.1 | + | 32171 | 0.66 | 0.970915 |
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Target: 5'- aCGUCgcgacccUCGAAGcccuUCCGGCCUCAcGCAa -3' miRNA: 3'- aGUAGa------AGCUUU----AGGUCGGGGU-CGUg -5' |
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| 13167 | 3' | -51.9 | NC_003401.1 | + | 95076 | 0.66 | 0.970915 |
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Target: 5'- uUCGUacagUCGGAAaaaaAGCUCCGGCACa -3' miRNA: 3'- -AGUAga--AGCUUUagg-UCGGGGUCGUG- -5' |
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| 13167 | 3' | -51.9 | NC_003401.1 | + | 39892 | 0.67 | 0.964461 |
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Target: 5'- aUCGUCUUUa-----CGGCCCCGGCGu -3' miRNA: 3'- -AGUAGAAGcuuuagGUCGGGGUCGUg -5' |
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| 13167 | 3' | -51.9 | NC_003401.1 | + | 96886 | 0.67 | 0.957065 |
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Target: 5'- cCAUCUUUGAuggccUCCA-CCCCAGUg- -3' miRNA: 3'- aGUAGAAGCUuu---AGGUcGGGGUCGug -5' |
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| 13167 | 3' | -51.9 | NC_003401.1 | + | 119516 | 0.67 | 0.946884 |
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Target: 5'- gUAUCgUCGGAcgCCGGCCggucaaaaagccaCAGCACg -3' miRNA: 3'- aGUAGaAGCUUuaGGUCGGg------------GUCGUG- -5' |
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| 13167 | 3' | -51.9 | NC_003401.1 | + | 123175 | 0.69 | 0.911201 |
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Target: 5'- cUCGUCUgucgggcCGAGG-CCAGUCCC-GCACc -3' miRNA: 3'- -AGUAGAa------GCUUUaGGUCGGGGuCGUG- -5' |
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| 13167 | 3' | -51.9 | NC_003401.1 | + | 102896 | 0.69 | 0.904801 |
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Target: 5'- -gAUCUggaaCGGAcgCguGCCCCAGUAUg -3' miRNA: 3'- agUAGAa---GCUUuaGguCGGGGUCGUG- -5' |
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| 13167 | 3' | -51.9 | NC_003401.1 | + | 15347 | 0.73 | 0.720512 |
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Target: 5'- cCAUCUUUGAAGUUCacgAGCCCCguGGCGg -3' miRNA: 3'- aGUAGAAGCUUUAGG---UCGGGG--UCGUg -5' |
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| 13167 | 3' | -51.9 | NC_003401.1 | + | 119515 | 0.73 | 0.710082 |
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Target: 5'- uUUAUUaUCGAGaggcuccgGUCCAGCCCCAGuUACu -3' miRNA: 3'- -AGUAGaAGCUU--------UAGGUCGGGGUC-GUG- -5' |
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| 13167 | 3' | -51.9 | NC_003401.1 | + | 126233 | 1.11 | 0.00373 |
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Target: 5'- gUCAUCUUCGAAAUCCAGCCCCAGCACg -3' miRNA: 3'- -AGUAGAAGCUUUAGGUCGGGGUCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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