Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13191 | 5' | -62.4 | NC_003401.1 | + | 17877 | 0.69 | 0.414387 |
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Target: 5'- -cGCCUcgCCGGagACGCgCAGGGGUccaccGGGCa -3' miRNA: 3'- gaCGGA--GGCU--UGCG-GUCCCCG-----UCCGc -5' |
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| 13191 | 5' | -62.4 | NC_003401.1 | + | 75095 | 0.69 | 0.405987 |
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Target: 5'- -cGCCgcCUGGGCGCuUGGcGGGCAGGCa -3' miRNA: 3'- gaCGGa-GGCUUGCG-GUC-CCCGUCCGc -5' |
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| 13191 | 5' | -62.4 | NC_003401.1 | + | 132646 | 0.69 | 0.405987 |
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Target: 5'- -gGUCUCCGcgcCGCCGGGaGGgGGGCc -3' miRNA: 3'- gaCGGAGGCuu-GCGGUCC-CCgUCCGc -5' |
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| 13191 | 5' | -62.4 | NC_003401.1 | + | 14334 | 0.69 | 0.397696 |
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Target: 5'- -cGgCUCCG-GCGCCggcAGGGGCGGGa- -3' miRNA: 3'- gaCgGAGGCuUGCGG---UCCCCGUCCgc -5' |
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| 13191 | 5' | -62.4 | NC_003401.1 | + | 117904 | 0.7 | 0.365639 |
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Target: 5'- -cGCCgaaCCGGGCG-CGGGGGUucGGCGg -3' miRNA: 3'- gaCGGa--GGCUUGCgGUCCCCGu-CCGC- -5' |
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| 13191 | 5' | -62.4 | NC_003401.1 | + | 14395 | 0.7 | 0.33541 |
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Target: 5'- cCUGCC-CCuGGCGUUcGGaGGCAGGCGg -3' miRNA: 3'- -GACGGaGGcUUGCGGuCC-CCGUCCGC- -5' |
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| 13191 | 5' | -62.4 | NC_003401.1 | + | 74933 | 0.74 | 0.217089 |
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Target: 5'- uCUGCCUgcCCGccaAGCGcCCAGGcGGCGGGCc -3' miRNA: 3'- -GACGGA--GGC---UUGC-GGUCC-CCGUCCGc -5' |
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| 13191 | 5' | -62.4 | NC_003401.1 | + | 53022 | 0.75 | 0.166316 |
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Target: 5'- uCUGCUgUCGuGCGCCGGGGGCccgcAGGCGc -3' miRNA: 3'- -GACGGaGGCuUGCGGUCCCCG----UCCGC- -5' |
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| 13191 | 5' | -62.4 | NC_003401.1 | + | 14238 | 1.09 | 0.000665 |
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Target: 5'- cCUGCCUCCGAACGCCAGGGGCAGGCGg -3' miRNA: 3'- -GACGGAGGCUUGCGGUCCCCGUCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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