Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13192 | 5' | -57.7 | NC_003401.1 | + | 116905 | 0.68 | 0.673495 |
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Target: 5'- -cGCAGgcaccugucCUGGGAGGACC-CAGCc--- -3' miRNA: 3'- gaCGUC---------GACCCUCCUGGaGUCGcaag -5' |
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| 13192 | 5' | -57.7 | NC_003401.1 | + | 111312 | 0.68 | 0.663215 |
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Target: 5'- -cGCcgaggaGGCUGGGcuuuuCCUCGGCGUUCg -3' miRNA: 3'- gaCG------UCGACCCuccu-GGAGUCGCAAG- -5' |
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| 13192 | 5' | -57.7 | NC_003401.1 | + | 21036 | 0.7 | 0.58076 |
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Target: 5'- gCUGCuucAGCUGcGGAGGACC-CcGCGUcCg -3' miRNA: 3'- -GACG---UCGAC-CCUCCUGGaGuCGCAaG- -5' |
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| 13192 | 5' | -57.7 | NC_003401.1 | + | 103328 | 0.71 | 0.471796 |
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Target: 5'- cCUGUGGCgUGGGuauGGGCgUCGGCGUg- -3' miRNA: 3'- -GACGUCG-ACCCu--CCUGgAGUCGCAag -5' |
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| 13192 | 5' | -57.7 | NC_003401.1 | + | 121474 | 0.72 | 0.453056 |
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Target: 5'- uUGCGGagggGGcGAGGACgUCGGCGUUg -3' miRNA: 3'- gACGUCga--CC-CUCCUGgAGUCGCAAg -5' |
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| 13192 | 5' | -57.7 | NC_003401.1 | + | 19975 | 0.72 | 0.416838 |
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Target: 5'- gUGCGGgaGGGGGGuguuugagccACCUguGUGUUCg -3' miRNA: 3'- gACGUCgaCCCUCC----------UGGAguCGCAAG- -5' |
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| 13192 | 5' | -57.7 | NC_003401.1 | + | 14934 | 1.1 | 0.001182 |
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Target: 5'- aCUGCAGCUGGGAGGACCUCAGCGUUCa -3' miRNA: 3'- -GACGUCGACCCUCCUGGAGUCGCAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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