Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13194 | 3' | -51.1 | NC_003401.1 | + | 20451 | 0.71 | 0.848807 |
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Target: 5'- ----gUCGGGGGaggcGCCAgcguagGGCUGGCCg -3' miRNA: 3'- ucuaaAGCCUCUau--CGGU------UCGACCGG- -5' |
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| 13194 | 3' | -51.1 | NC_003401.1 | + | 106823 | 0.74 | 0.725288 |
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Target: 5'- aAGAUucccuuuggUUCGGuGAccUGGCCGgaGGCUGGCUg -3' miRNA: 3'- -UCUA---------AAGCCuCU--AUCGGU--UCGACCGG- -5' |
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| 13194 | 3' | -51.1 | NC_003401.1 | + | 14205 | 0.75 | 0.672535 |
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Target: 5'- aGGGUgUCGGGGAccGGCCGGGUgcgcgaaucgauUGGCCg -3' miRNA: 3'- -UCUAaAGCCUCUa-UCGGUUCG------------ACCGG- -5' |
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| 13194 | 3' | -51.1 | NC_003401.1 | + | 103903 | 0.77 | 0.576065 |
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Target: 5'- uGGGUUUCGGGGG-AGUCAcgacGUUGGCCg -3' miRNA: 3'- -UCUAAAGCCUCUaUCGGUu---CGACCGG- -5' |
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| 13194 | 3' | -51.1 | NC_003401.1 | + | 15655 | 1.14 | 0.003321 |
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Target: 5'- gAGAUUUCGGAGAUAGCCAAGCUGGCCa -3' miRNA: 3'- -UCUAAAGCCUCUAUCGGUUCGACCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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