Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
132 | 3' | -59.9 | AC_000006.1 | + | 14993 | 0.66 | 0.377122 |
Target: 5'- cGCuGGACUcCGCGGauacugcuGCGcAGCGGuaGCg -3' miRNA: 3'- cCGuCCUGA-GCGUC--------UGC-UCGCCggCG- -5' |
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132 | 3' | -59.9 | AC_000006.1 | + | 24434 | 0.66 | 0.368408 |
Target: 5'- aGCuGGGGgUUGCAGACcagGAGCGG-UGCg -3' miRNA: 3'- cCG-UCCUgAGCGUCUG---CUCGCCgGCG- -5' |
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132 | 3' | -59.9 | AC_000006.1 | + | 30605 | 0.66 | 0.359833 |
Target: 5'- cGCAGGA---GCAGugGAuGCaGCCGUu -3' miRNA: 3'- cCGUCCUgagCGUCugCU-CGcCGGCG- -5' |
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132 | 3' | -59.9 | AC_000006.1 | + | 5315 | 0.66 | 0.351399 |
Target: 5'- aGGCGGGACagaggagggaUUGCAGGgcguaGAGCuuGGgCGCa -3' miRNA: 3'- -CCGUCCUG----------AGCGUCUg----CUCG--CCgGCG- -5' |
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132 | 3' | -59.9 | AC_000006.1 | + | 13107 | 0.66 | 0.351399 |
Target: 5'- uGCcGGGCUCugugaacagcgGCAGgguGAGcCGGCCGCg -3' miRNA: 3'- cCGuCCUGAG-----------CGUCug-CUC-GCCGGCG- -5' |
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132 | 3' | -59.9 | AC_000006.1 | + | 15584 | 0.66 | 0.343105 |
Target: 5'- uGGCcGcGGCg-GCGG-CG-GCGGCCGCu -3' miRNA: 3'- -CCGuC-CUGagCGUCuGCuCGCCGGCG- -5' |
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132 | 3' | -59.9 | AC_000006.1 | + | 9654 | 0.67 | 0.338197 |
Target: 5'- aGGCGGaGuCUuccaUGCGGGugaccccgacgccccUGAGCGGCUGCa -3' miRNA: 3'- -CCGUC-CuGA----GCGUCU---------------GCUCGCCGGCG- -5' |
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132 | 3' | -59.9 | AC_000006.1 | + | 8077 | 0.67 | 0.303775 |
Target: 5'- cGGCgGGGGCggagagCGaAGACGAG-GGCgCGCa -3' miRNA: 3'- -CCG-UCCUGa-----GCgUCUGCUCgCCG-GCG- -5' |
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132 | 3' | -59.9 | AC_000006.1 | + | 16770 | 0.67 | 0.296338 |
Target: 5'- cGCAGGAgaggcaUgGCAGGC-AGCGGCCu- -3' miRNA: 3'- cCGUCCUg-----AgCGUCUGcUCGCCGGcg -5' |
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132 | 3' | -59.9 | AC_000006.1 | + | 10244 | 0.68 | 0.289042 |
Target: 5'- uGGC-GGAa-CGCAaACGGGUuaGGCCGCg -3' miRNA: 3'- -CCGuCCUgaGCGUcUGCUCG--CCGGCG- -5' |
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132 | 3' | -59.9 | AC_000006.1 | + | 23156 | 0.68 | 0.286881 |
Target: 5'- gGGUuGGGCUCGCAGgugccguugggcugGCGuugGGgGGCaCGCg -3' miRNA: 3'- -CCGuCCUGAGCGUC--------------UGC---UCgCCG-GCG- -5' |
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132 | 3' | -59.9 | AC_000006.1 | + | 24313 | 0.68 | 0.274876 |
Target: 5'- --gAGGACgugaGCGG-CGAGgGGCUGCu -3' miRNA: 3'- ccgUCCUGag--CGUCuGCUCgCCGGCG- -5' |
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132 | 3' | -59.9 | AC_000006.1 | + | 13778 | 0.68 | 0.274183 |
Target: 5'- aGGCua-GCaCGCAgacgauaaauuuuGACGAGCGGUCGCg -3' miRNA: 3'- -CCGuccUGaGCGU-------------CUGCUCGCCGGCG- -5' |
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132 | 3' | -59.9 | AC_000006.1 | + | 5399 | 0.69 | 0.241899 |
Target: 5'- gGGCGcagacGGuCUCGCAcucGACGAGCcaggugagcucgGGCUGCu -3' miRNA: 3'- -CCGU-----CCuGAGCGU---CUGCUCG------------CCGGCG- -5' |
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132 | 3' | -59.9 | AC_000006.1 | + | 10096 | 0.69 | 0.223737 |
Target: 5'- cGCGGGaACUCGCGGAC--GCGGUuccagauguugCGCa -3' miRNA: 3'- cCGUCC-UGAGCGUCUGcuCGCCG-----------GCG- -5' |
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132 | 3' | -59.9 | AC_000006.1 | + | 16639 | 0.7 | 0.205112 |
Target: 5'- gGGCGGGGg--GCAGACGGcggcggguggcgguGCGGCgGCg -3' miRNA: 3'- -CCGUCCUgagCGUCUGCU--------------CGCCGgCG- -5' |
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132 | 3' | -59.9 | AC_000006.1 | + | 15541 | 0.7 | 0.196051 |
Target: 5'- uGCGGGGggUGCAGugGcggcGCGGCgCGCg -3' miRNA: 3'- cCGUCCUgaGCGUCugCu---CGCCG-GCG- -5' |
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132 | 3' | -59.9 | AC_000006.1 | + | 10392 | 0.7 | 0.185841 |
Target: 5'- cGGCcganGGGAgUCGCuAGACuugaaAGCGGCCGa -3' miRNA: 3'- -CCG----UCCUgAGCG-UCUGc----UCGCCGGCg -5' |
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132 | 3' | -59.9 | AC_000006.1 | + | 10489 | 0.7 | 0.185841 |
Target: 5'- cGGCAGaACccgguUCGCGGACGGccGCGGCgaGCg -3' miRNA: 3'- -CCGUCcUG-----AGCGUCUGCU--CGCCGg-CG- -5' |
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132 | 3' | -59.9 | AC_000006.1 | + | 17644 | 0.71 | 0.15798 |
Target: 5'- gGGCGGGGa-CGCGG-CGcacGGCGGUCGCc -3' miRNA: 3'- -CCGUCCUgaGCGUCuGC---UCGCCGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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