Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13201 | 5' | -61.7 | NC_003401.1 | + | 47661 | 0.66 | 0.651249 |
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Target: 5'- -gAUCCGUCGcgGGUC-AGUGCGaCGGGu -3' miRNA: 3'- caUGGGCGGCa-CCAGcUCGCGC-GCCU- -5' |
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| 13201 | 5' | -61.7 | NC_003401.1 | + | 9231 | 0.66 | 0.631491 |
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Target: 5'- -gGCgUGCCGUGGacuaUCGAG-GaCGCGGGu -3' miRNA: 3'- caUGgGCGGCACC----AGCUCgC-GCGCCU- -5' |
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| 13201 | 5' | -61.7 | NC_003401.1 | + | 44025 | 0.66 | 0.62161 |
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Target: 5'- --cCCCGuuG-GGUCGAGCucaGCuauGCGGAu -3' miRNA: 3'- cauGGGCggCaCCAGCUCG---CG---CGCCU- -5' |
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| 13201 | 5' | -61.7 | NC_003401.1 | + | 132445 | 0.67 | 0.572447 |
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Target: 5'- -cGCCCGgCGcgcgGGcCcgggGGGCGCGCGGGg -3' miRNA: 3'- caUGGGCgGCa---CCaG----CUCGCGCGCCU- -5' |
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| 13201 | 5' | -61.7 | NC_003401.1 | + | 32318 | 0.68 | 0.496089 |
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Target: 5'- gGUGCCCGCCuaaaagggcgGUGGUgcCGGGCG-GUGGc -3' miRNA: 3'- -CAUGGGCGG----------CACCA--GCUCGCgCGCCu -5' |
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| 13201 | 5' | -61.7 | NC_003401.1 | + | 109614 | 0.7 | 0.397046 |
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Target: 5'- -gGCCCGCCcuucggGGUCGGaguugaaggauauuGgGCGCGGAg -3' miRNA: 3'- caUGGGCGGca----CCAGCU--------------CgCGCGCCU- -5' |
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| 13201 | 5' | -61.7 | NC_003401.1 | + | 17544 | 0.7 | 0.391336 |
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Target: 5'- -cGCCCGCUgaccguGUGGguaaacgCGAGCGgGCGGc -3' miRNA: 3'- caUGGGCGG------CACCa------GCUCGCgCGCCu -5' |
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| 13201 | 5' | -61.7 | NC_003401.1 | + | 21606 | 0.77 | 0.141225 |
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Target: 5'- -aGCUCGCCGUGGUCGccgcggcacGCGCaGCGGAc -3' miRNA: 3'- caUGGGCGGCACCAGCu--------CGCG-CGCCU- -5' |
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| 13201 | 5' | -61.7 | NC_003401.1 | + | 18804 | 1.08 | 0.001001 |
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Target: 5'- aGUACCCGCCGUGGUCGAGCGCGCGGAu -3' miRNA: 3'- -CAUGGGCGGCACCAGCUCGCGCGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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