miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1321 3' -58.7 NC_001331.1 + 6860 1.11 0.000019
Target:  5'- gCCCGCACCGUGGCCGAUCCGGUCAAGg -3'
miRNA:   3'- -GGGCGUGGCACCGGCUAGGCCAGUUC- -5'
1321 3' -58.7 NC_001331.1 + 1932 0.69 0.064225
Target:  5'- gCCCGC-CC--GGCUGGUCgUGGUCGGGg -3'
miRNA:   3'- -GGGCGuGGcaCCGGCUAG-GCCAGUUC- -5'
1321 3' -58.7 NC_001331.1 + 657 0.66 0.113435
Target:  5'- uUCCGC-CCGaGGCCGcuggauacguggacAUCCuGGUCAAc -3'
miRNA:   3'- -GGGCGuGGCaCCGGC--------------UAGG-CCAGUUc -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.