Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13213 | 5' | -59.2 | NC_003401.1 | + | 90577 | 0.66 | 0.673495 |
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Target: 5'- uGCGU--UCCUGGCCCcaaGACGUGCGu -3' miRNA: 3'- uUGCAccGGGACCGGGa--CUGCAUGUu -5' |
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| 13213 | 5' | -59.2 | NC_003401.1 | + | 14284 | 0.66 | 0.663215 |
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Target: 5'- gGGCGUGGUCCgcgagGGCCC--AgGUACGGg -3' miRNA: 3'- -UUGCACCGGGa----CCGGGacUgCAUGUU- -5' |
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| 13213 | 5' | -59.2 | NC_003401.1 | + | 57179 | 0.67 | 0.650846 |
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Target: 5'- gAACGgGGCCCUGGCCgaauuuuuuagGGCGcUGCAc -3' miRNA: 3'- -UUGCaCCGGGACCGGga---------CUGC-AUGUu -5' |
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| 13213 | 5' | -59.2 | NC_003401.1 | + | 105208 | 0.67 | 0.632257 |
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Target: 5'- cAUGUGGCCCa--CCCUGGCGgccGCAAu -3' miRNA: 3'- uUGCACCGGGaccGGGACUGCa--UGUU- -5' |
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| 13213 | 5' | -59.2 | NC_003401.1 | + | 92512 | 0.68 | 0.591013 |
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Target: 5'- gGACGggGGCCCU-GCCCUGGCcgcugaucuGUAUAAc -3' miRNA: 3'- -UUGCa-CCGGGAcCGGGACUG---------CAUGUU- -5' |
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| 13213 | 5' | -59.2 | NC_003401.1 | + | 121348 | 0.68 | 0.550252 |
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Target: 5'- aAACGggGGUCCUGGCCgaGACGg---- -3' miRNA: 3'- -UUGCa-CCGGGACCGGgaCUGCauguu -5' |
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| 13213 | 5' | -59.2 | NC_003401.1 | + | 41765 | 1.03 | 0.002451 |
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Target: 5'- cAACGUGGCCCUGGCCCUGACGUACAAc -3' miRNA: 3'- -UUGCACCGGGACCGGGACUGCAUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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