Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1322 | 5' | -64.8 | NC_001331.1 | + | 2759 | 0.66 | 0.049341 |
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Target: 5'- aGCauguaugCCGCGCUgCCGGGUcCgauugCGGCGg -3' miRNA: 3'- gCGg------GGCGCGAgGGCUCGuGa----GCCGC- -5' |
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| 1322 | 5' | -64.8 | NC_001331.1 | + | 107 | 0.66 | 0.043444 |
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Target: 5'- uCGCCCCGa--UCCCGgaaGGCGCccgUGGCa -3' miRNA: 3'- -GCGGGGCgcgAGGGC---UCGUGa--GCCGc -5' |
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| 1322 | 5' | -64.8 | NC_001331.1 | + | 7042 | 0.67 | 0.037034 |
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Target: 5'- cCGCCgaGUGCUCggGAGCGCggGGCGg -3' miRNA: 3'- -GCGGggCGCGAGggCUCGUGagCCGC- -5' |
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| 1322 | 5' | -64.8 | NC_001331.1 | + | 4060 | 0.67 | 0.035869 |
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Target: 5'- gGCCaCCGCGCcggccugccUCCCG-GCACa-GGUGa -3' miRNA: 3'- gCGG-GGCGCG---------AGGGCuCGUGagCCGC- -5' |
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| 1322 | 5' | -64.8 | NC_001331.1 | + | 3885 | 0.69 | 0.026024 |
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Target: 5'- uCGCCCCGCGaCUUgCGguaGGCAUg-GGCGa -3' miRNA: 3'- -GCGGGGCGC-GAGgGC---UCGUGagCCGC- -5' |
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| 1322 | 5' | -64.8 | NC_001331.1 | + | 4311 | 0.75 | 0.006473 |
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Target: 5'- uGCCuuGCGCUCCCG-GCcggCGGCGc -3' miRNA: 3'- gCGGggCGCGAGGGCuCGugaGCCGC- -5' |
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| 1322 | 5' | -64.8 | NC_001331.1 | + | 7008 | 1.08 | 0.000006 |
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Target: 5'- cCGCCCCGCGCUCCCGAGCACUCGGCGg -3' miRNA: 3'- -GCGGGGCGCGAGGGCUCGUGAGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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