miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1322 5' -64.8 NC_001331.1 + 2759 0.66 0.049341
Target:  5'- aGCauguaugCCGCGCUgCCGGGUcCgauugCGGCGg -3'
miRNA:   3'- gCGg------GGCGCGAgGGCUCGuGa----GCCGC- -5'
1322 5' -64.8 NC_001331.1 + 107 0.66 0.043444
Target:  5'- uCGCCCCGa--UCCCGgaaGGCGCccgUGGCa -3'
miRNA:   3'- -GCGGGGCgcgAGGGC---UCGUGa--GCCGc -5'
1322 5' -64.8 NC_001331.1 + 7042 0.67 0.037034
Target:  5'- cCGCCgaGUGCUCggGAGCGCggGGCGg -3'
miRNA:   3'- -GCGGggCGCGAGggCUCGUGagCCGC- -5'
1322 5' -64.8 NC_001331.1 + 4060 0.67 0.035869
Target:  5'- gGCCaCCGCGCcggccugccUCCCG-GCACa-GGUGa -3'
miRNA:   3'- gCGG-GGCGCG---------AGGGCuCGUGagCCGC- -5'
1322 5' -64.8 NC_001331.1 + 3885 0.69 0.026024
Target:  5'- uCGCCCCGCGaCUUgCGguaGGCAUg-GGCGa -3'
miRNA:   3'- -GCGGGGCGC-GAGgGC---UCGUGagCCGC- -5'
1322 5' -64.8 NC_001331.1 + 4311 0.75 0.006473
Target:  5'- uGCCuuGCGCUCCCG-GCcggCGGCGc -3'
miRNA:   3'- gCGGggCGCGAGGGCuCGugaGCCGC- -5'
1322 5' -64.8 NC_001331.1 + 7008 1.08 0.000006
Target:  5'- cCGCCCCGCGCUCCCGAGCACUCGGCGg -3'
miRNA:   3'- -GCGGGGCGCGAGGGCUCGUGAGCCGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.