Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13233 | 3' | -44.8 | NC_003401.1 | + | 19996 | 0.66 | 0.999996 |
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Target: 5'- aACugGuuuUgUGUGGCGAGG-GUGcgGGa -3' miRNA: 3'- -UGugUu--AgACACUGCUCUaCAUuaCC- -5' |
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| 13233 | 3' | -44.8 | NC_003401.1 | + | 57146 | 0.66 | 0.999988 |
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Target: 5'- uCACGuucUCUGggugGACGAGGcgUGUAuGUGGa -3' miRNA: 3'- uGUGUu--AGACa---CUGCUCU--ACAU-UACC- -5' |
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| 13233 | 3' | -44.8 | NC_003401.1 | + | 82356 | 0.66 | 0.999988 |
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Target: 5'- gGCACGaguGUCUGUGGCGGGcguUGUc---- -3' miRNA: 3'- -UGUGU---UAGACACUGCUCu--ACAuuacc -5' |
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| 13233 | 3' | -44.8 | NC_003401.1 | + | 70528 | 0.67 | 0.999976 |
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Target: 5'- -gGCGGuUCUGUGucaGGGAUGU-AUGGa -3' miRNA: 3'- ugUGUU-AGACACug-CUCUACAuUACC- -5' |
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| 13233 | 3' | -44.8 | NC_003401.1 | + | 28050 | 0.68 | 0.99992 |
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Target: 5'- uGCGCu-UCUgGUGGCGAGAcgGUGccGGa -3' miRNA: 3'- -UGUGuuAGA-CACUGCUCUa-CAUuaCC- -5' |
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| 13233 | 3' | -44.8 | NC_003401.1 | + | 121653 | 0.7 | 0.998848 |
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Target: 5'- gGC-CGGggUGUGACGGGG-GUGAUGGu -3' miRNA: 3'- -UGuGUUagACACUGCUCUaCAUUACC- -5' |
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| 13233 | 3' | -44.8 | NC_003401.1 | + | 63949 | 0.73 | 0.988711 |
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Target: 5'- aACGCGGUCaGUGGCGcgggcgcgcucgguGGUGUGGUGGc -3' miRNA: 3'- -UGUGUUAGaCACUGCu-------------CUACAUUACC- -5' |
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| 13233 | 3' | -44.8 | NC_003401.1 | + | 87982 | 0.76 | 0.9541 |
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Target: 5'- gGCACGaguGUCUGUGGCGGGc-GUGcgGGg -3' miRNA: 3'- -UGUGU---UAGACACUGCUCuaCAUuaCC- -5' |
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| 13233 | 3' | -44.8 | NC_003401.1 | + | 95470 | 1.13 | 0.020102 |
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Target: 5'- cACACAAUCUGUGACGAGAUGUAAUGGa -3' miRNA: 3'- -UGUGUUAGACACUGCUCUACAUUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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