Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13235 | 5' | -47.4 | NC_003401.1 | + | 26746 | 0.66 | 0.999376 |
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Target: 5'- cCGCGGUGCggcauacgguUUAAcguGCAACUGCcg-GUCc -3' miRNA: 3'- -GCGUCACG----------AAUU---CGUUGACGcaaUAG- -5' |
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| 13235 | 5' | -47.4 | NC_003401.1 | + | 10056 | 0.66 | 0.999016 |
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Target: 5'- uCGCGGUGUU--GGCAgaccgacACUGCG--AUCa -3' miRNA: 3'- -GCGUCACGAauUCGU-------UGACGCaaUAG- -5' |
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| 13235 | 5' | -47.4 | NC_003401.1 | + | 126452 | 0.66 | 0.998815 |
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Target: 5'- gGCGGUgGCcgUGAuGaCGACUGCGUUAa- -3' miRNA: 3'- gCGUCA-CGa-AUU-C-GUUGACGCAAUag -5' |
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| 13235 | 5' | -47.4 | NC_003401.1 | + | 110199 | 0.67 | 0.997445 |
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Target: 5'- uGCAGUGCUcacagUGAGCuGACcGCGUc--- -3' miRNA: 3'- gCGUCACGA-----AUUCG-UUGaCGCAauag -5' |
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| 13235 | 5' | -47.4 | NC_003401.1 | + | 114843 | 0.67 | 0.997445 |
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Target: 5'- uGUGGUGCUUGGuGCAACggugGUGggGUg -3' miRNA: 3'- gCGUCACGAAUU-CGUUGa---CGCaaUAg -5' |
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| 13235 | 5' | -47.4 | NC_003401.1 | + | 110083 | 0.68 | 0.996371 |
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Target: 5'- uGCAccGUGCgccugcAGCAcgGCUGCGUcUAUCa -3' miRNA: 3'- gCGU--CACGaau---UCGU--UGACGCA-AUAG- -5' |
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| 13235 | 5' | -47.4 | NC_003401.1 | + | 68152 | 0.68 | 0.994951 |
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Target: 5'- cCGCAGUGUcgGAGCAACaUG-GUuuacUGUCu -3' miRNA: 3'- -GCGUCACGaaUUCGUUG-ACgCA----AUAG- -5' |
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| 13235 | 5' | -47.4 | NC_003401.1 | + | 100125 | 0.78 | 0.720424 |
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Target: 5'- aCGCAGuUGCUUAAGC-ACUGCGa---- -3' miRNA: 3'- -GCGUC-ACGAAUUCGuUGACGCaauag -5' |
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| 13235 | 5' | -47.4 | NC_003401.1 | + | 99970 | 1.11 | 0.011262 |
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Target: 5'- uCGCAGUGCUUAAGCAACUGCGUUAUCu -3' miRNA: 3'- -GCGUCACGAAUUCGUUGACGCAAUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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