Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 13239 | 3' | -63.3 | NC_003401.1 | + | 107791 | 0.69 | 0.401828 |
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Target: 5'- -gGGCcccGCGCCCGGUGGcgcGGACcCGGg -3' miRNA: 3'- ggCCG---CGCGGGCCGCUa--CCUGaGCCa -5' |
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| 13239 | 3' | -63.3 | NC_003401.1 | + | 128035 | 0.71 | 0.305525 |
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Target: 5'- gCUGGCGCGCaCCGGCacgccUGucCUCGGUg -3' miRNA: 3'- -GGCCGCGCG-GGCCGcu---ACcuGAGCCA- -5' |
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| 13239 | 3' | -63.3 | NC_003401.1 | + | 132533 | 0.72 | 0.261462 |
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Target: 5'- cCCGGCGCGC--GGCGcgGGGCggcCGGg -3' miRNA: 3'- -GGCCGCGCGggCCGCuaCCUGa--GCCa -5' |
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| 13239 | 3' | -63.3 | NC_003401.1 | + | 132677 | 0.72 | 0.292381 |
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Target: 5'- cCCGGgGCGCCuCGGCG--GGGCcCGGc -3' miRNA: 3'- -GGCCgCGCGG-GCCGCuaCCUGaGCCa -5' |
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| 13239 | 3' | -63.3 | NC_003401.1 | + | 133711 | 0.73 | 0.222714 |
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Target: 5'- gCCGG-GUGCCCGGCGGUccGGGCggGGg -3' miRNA: 3'- -GGCCgCGCGGGCCGCUA--CCUGagCCa -5' |
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| 13239 | 3' | -63.3 | NC_003401.1 | + | 103308 | 0.74 | 0.216583 |
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Target: 5'- gUCGGCGUGUaguccaugggaaCGGCGGUGGcCUCGGa -3' miRNA: 3'- -GGCCGCGCGg-----------GCCGCUACCuGAGCCa -5' |
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| 13239 | 3' | -63.3 | NC_003401.1 | + | 132453 | 0.8 | 0.083912 |
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Target: 5'- gCCGGCgGCGCCCGGCGcgcGGGCcCGGg -3' miRNA: 3'- -GGCCG-CGCGGGCCGCua-CCUGaGCCa -5' |
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| 13239 | 3' | -63.3 | NC_003401.1 | + | 104678 | 1.08 | 0.000757 |
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Target: 5'- uCCGGCGCGCCCGGCGAUGGACUCGGUg -3' miRNA: 3'- -GGCCGCGCGGGCCGCUACCUGAGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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