Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13239 | 3' | -63.3 | NC_003401.1 | + | 23704 | 0.66 | 0.552883 |
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Target: 5'- aCCGGCaGCG-CCGGU--UGGACgcggCGGa -3' miRNA: 3'- -GGCCG-CGCgGGCCGcuACCUGa---GCCa -5' |
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| 13239 | 3' | -63.3 | NC_003401.1 | + | 21359 | 0.67 | 0.519035 |
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Target: 5'- cCCGuGCGCgucccaaauuuuuacGCCgCGGCGcgacaguucgGUGGAgUCGGUg -3' miRNA: 3'- -GGC-CGCG---------------CGG-GCCGC----------UACCUgAGCCA- -5' |
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| 13239 | 3' | -63.3 | NC_003401.1 | + | 20281 | 0.68 | 0.469912 |
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Target: 5'- aCGaCGCGCCCGGgGAcgcGGACgccgCGGc -3' miRNA: 3'- gGCcGCGCGGGCCgCUa--CCUGa---GCCa -5' |
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| 13239 | 3' | -63.3 | NC_003401.1 | + | 19405 | 0.66 | 0.610575 |
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Target: 5'- uUGGCGCauuaCCGGUGAgGGACagGGUc -3' miRNA: 3'- gGCCGCGcg--GGCCGCUaCCUGagCCA- -5' |
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| 13239 | 3' | -63.3 | NC_003401.1 | + | 15588 | 0.67 | 0.515323 |
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Target: 5'- -gGGCaGCuaUUGcGUGAUGGACUCGGUc -3' miRNA: 3'- ggCCG-CGcgGGC-CGCUACCUGAGCCA- -5' |
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| 13239 | 3' | -63.3 | NC_003401.1 | + | 13250 | 0.68 | 0.452324 |
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Target: 5'- -gGGCGCGaguagcagaucCCCGG-GGUGGACgugCGGa -3' miRNA: 3'- ggCCGCGC-----------GGGCCgCUACCUGa--GCCa -5' |
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| 13239 | 3' | -63.3 | NC_003401.1 | + | 11777 | 0.66 | 0.610575 |
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Target: 5'- -gGGCGUcgacggGCCCGGCGuUGG-CUucgCGGUg -3' miRNA: 3'- ggCCGCG------CGGGCCGCuACCuGA---GCCA- -5' |
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| 13239 | 3' | -63.3 | NC_003401.1 | + | 9522 | 0.67 | 0.533986 |
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Target: 5'- cCCGGCcaguaGgGCgCCGGCGcuaGGGgUCGGUa -3' miRNA: 3'- -GGCCG-----CgCG-GGCCGCua-CCUgAGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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