Results 41 - 43 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13239 | 5' | -53.5 | NC_003401.1 | + | 21778 | 0.72 | 0.687317 |
Target: 5'- cGGCCGucCGCUg-CGCGUGcCGCGGCg -3' miRNA: 3'- aCUGGUuaGUGGaaGUGCAC-GCGCCG- -5' |
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13239 | 5' | -53.5 | NC_003401.1 | + | 93318 | 0.77 | 0.416497 |
Target: 5'- gGACUuuuUCGCCUUCGCGgaaucaUGCGCGGg -3' miRNA: 3'- aCUGGuu-AGUGGAAGUGC------ACGCGCCg -5' |
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13239 | 5' | -53.5 | NC_003401.1 | + | 42748 | 0.66 | 0.960922 |
Target: 5'- aUGuCCGAgguaaUCGCCgcaACGUGgGCGGg -3' miRNA: 3'- -ACuGGUU-----AGUGGaagUGCACgCGCCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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