Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13240 | 3' | -56 | NC_003401.1 | + | 13673 | 0.66 | 0.878216 |
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Target: 5'- uCGGACAGgucgcgGGCCaGGCGgUCGCgguUCAGGu -3' miRNA: 3'- -GUCUGUCa-----CCGGgCUGU-AGCG---AGUCU- -5' |
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| 13240 | 3' | -56 | NC_003401.1 | + | 111632 | 0.68 | 0.795871 |
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Target: 5'- -uGACAGUGaGUCCGG-AUCGUUUAGGa -3' miRNA: 3'- guCUGUCAC-CGGGCUgUAGCGAGUCU- -5' |
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| 13240 | 3' | -56 | NC_003401.1 | + | 13711 | 0.69 | 0.748525 |
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Target: 5'- -cGACcGUGcGgCCGACAUCGCcCAGGu -3' miRNA: 3'- guCUGuCAC-CgGGCUGUAGCGaGUCU- -5' |
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| 13240 | 3' | -56 | NC_003401.1 | + | 63952 | 0.69 | 0.718786 |
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Target: 5'- gCGGuCAGUGGCgCgGGCG-CGCUCGGu -3' miRNA: 3'- -GUCuGUCACCG-GgCUGUaGCGAGUCu -5' |
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| 13240 | 3' | -56 | NC_003401.1 | + | 16774 | 0.7 | 0.688365 |
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Target: 5'- cCAGGucCAGgaUGGCgCGGCAUCGCUCcuGGAc -3' miRNA: 3'- -GUCU--GUC--ACCGgGCUGUAGCGAG--UCU- -5' |
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| 13240 | 3' | -56 | NC_003401.1 | + | 111438 | 0.7 | 0.678116 |
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Target: 5'- uCAGGCAGgGGCCCuGugAUUGgUCAGc -3' miRNA: 3'- -GUCUGUCaCCGGG-CugUAGCgAGUCu -5' |
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| 13240 | 3' | -56 | NC_003401.1 | + | 103286 | 0.71 | 0.636824 |
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Target: 5'- -cGGCGGUGGCCUcgGACGgcggUCGCUCGu- -3' miRNA: 3'- guCUGUCACCGGG--CUGU----AGCGAGUcu -5' |
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| 13240 | 3' | -56 | NC_003401.1 | + | 104970 | 1.09 | 0.00252 |
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Target: 5'- aCAGACAGUGGCCCGACAUCGCUCAGAc -3' miRNA: 3'- -GUCUGUCACCGGGCUGUAGCGAGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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